Aeromonas Medium Base (Ryan) (Dehydrated)

Isolate Aeromonas hydrophila from clinical and environmental specimens with selective, diagnostic Thermo Scientific™ Oxoid™ Aeromonas Medium Base (Ryan) (Dehydrated) when used with Ampicillin Selective Supplement (SR0136).

Ryan¹ modified the formulation of XLD Medium so that it would support the growth of Aeromonas spp and Plesiomonas spp as well as the usual Enterobacteriaceae. It could therefore be used as a universal medium in the investigation of enteric disease. To improve its performance in the isolation of aeromonads, the addition of ampicillin at 5mg/L is recommended.

Aeromonas spp occur widely in soil and water, where they cause diseases in fish and amphibians. They also occur in untreated and chlorinated drinking water, raw foods and raw milk.^2,3It is considered that the major cause of gastrointestinal infections by Aeromonas spp^3,4 is from ingesting infected water.^4,5

Aeromonas Medium Base has been developed to improve the enumeration and isolation of Aeromonas spp from clinical and environmental specimens.

• Specified by the MAFF/DHS Steering Group on the Microbiological Safety of Food for detection and enumeration of Aeromonas hydrophilain clinical specimens⁷.
• Superior performance over other formulae for detection of Aeromonas in tap water, bottled water and foods including meat, poultry, fish and seafoods^8,9,10

To improve the performance of the medium in the isolation of aeromonads, the addition of ampicillin at 5mg/L is recommended. The effectiveness of ampicillin as a selective agent for Aeromonas spp has been reported by several workers ^{11,12,13,14,15}. The value of Aeromonas Medium Base (Ryan) is that the recommended level of ampicillin is well below that which can cause inhibition of some strains of aeromonad¹⁶.

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