FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

Citations & References (4)

Invitrogen™

FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-solubleRead more

Catalog Number	Quantity
F10317	1 mg

Catalog number F10317

Price (EUR)

632,00

Add to cart

Quantity:

Price (EUR)

632,00

Add to cart

FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-soluble FM® dyes are virtually nonfluorescent in aqueous media and are believed to insert into the surface membrane where they become intensely fluorescent. FM® 1-43 stain has been used successfully to stain the cell bodies specifically in a complex biofilm mileu, including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus sp., Acidothiobacillus caldus, and Vibrio cholerae.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorGreen

Detection MethodFluorescence

Dye TypeFM 1-43⁄lipid

EmissionVisible

Excitation Wavelength Range472⁄580

For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, Flow Cytometer, Microplate Reader, Fluorescent Imager

FormSolid

Product LineFilmTracer, Molecular Probes

Quantity1 mg

Shipping ConditionRoom Temperature

Label TypeOther Labels or Dyes

Product TypeDye

SubCellular LocalizationCell Membranes, Lipids

Unit SizeEach

Contents & Storage

Store at room temperature; Dessicate; Protect from light

Frequently asked questions (FAQs)

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References

Abstract

A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm.

Authors:Houot L, Watnick PI,

Journal:J Bacteriol

PubMed ID:17981973

Glucose is a universal energy source and a potent inducer of surface colonization for many microbial species. Highly efficient sugar assimilation pathways ensure successful competition for this preferred carbon source. One such pathway is the phosphoenolpyruvate phosphotransferase system (PTS), a multicomponent sugar transport system that phosphorylates the sugar as it ... More

Fluorescence staining of live cyanobacterial cells suggest non-stringent chromosome segregation and absence of a connection between cytoplasmic and thylakoid membranes.

Authors:Schneider D, Fuhrmann E, Scholz I, Hess WR, Graumann PL,

Journal:BMC Cell Biol

PubMed ID:17767716

BACKGROUND: In spite of their abundance and importance, little is known about cyanobacterial cell biology and their cell cycle. During each cell cycle, chromosomes must be separated into future daughter cells, i.e. into both cell halves, which in many bacteria is achieved by an active machinery that operates during DNA ... More

Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community.

Authors:

Journal:Appl Environ Microbiol

PubMed ID:28754701

Beta-Defensin 2 and 3 Promote Bacterial Clearance of Pseudomonas aeruginosa by Inhibiting Macrophage Autophagy through Downregulation of Early Growth Response Gene-1 and c-FOS.

Authors:

Journal:Front Immunol

PubMed ID:29487594