FM™ 1-43FX, fixable analog of FM™ 1-43 membrane stain
FM™ 1-43FX, fixable analog of FM™ 1-43 membrane stain
Citations & References (35)
Invitrogen™

FM™ 1-43FX, fixable analog of FM™ 1-43 membrane stain

FM 1-43FX membrane probe is an FM 1-43 membrane probe analog that has been modified to contain an aliphatic amine.Read more
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Catalog NumberQuantity
F3535510 x 100 μg
Catalog number F35355
Price (EUR)
835,65
온라인 행사
892,00
Save 56,35 (6%)
Each
Add to cart
Quantity:
10 x 100 μg
Price (EUR)
835,65
온라인 행사
892,00
Save 56,35 (6%)
Each
Add to cart
FM 1-43FX membrane probe is an FM 1-43 membrane probe analog that has been modified to contain an aliphatic amine. That modification makes the probe fixable with aldehyde-based fixatives (formaldehyde, glutaraldehyde, etc.). FM 1-43 membrane probe is widely used both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. Unmodified FM 1-43 membrane probe is available as 1 mg in a single vial (T-3163) and as 1 mg packaged into 10 vials, each containing 100 μg (T-35356).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineFM
Quantity10 x 100 μg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeMembrane Stain
SubCellular LocalizationPlasma Membrane
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (35)

Citations & References
Abstract
A developmental switch in neurotransmitter flux enhances synaptic efficacy by affecting AMPA receptor activation.
Authors:Renger JJ, Egles C, Liu G
Journal:Neuron
PubMed ID:11239436
'Formation of glutamatergic synapses entails development of "silent" immature contacts into mature functional synapses. To determine how this transformation occurs, we investigated the development of neurotransmission at single synapses in vitro. Maturation of presynaptic function, assayed with endocytotic markers, followed accumulation of synapsin I. During this period, synaptic transmission was ... More
Directed cell growth on protein-functionalized hydrogel surfaces.
Authors:Hynd MR, Frampton JP, Dowell-Mesfin N, Turner JN, Shain W
Journal:J Neurosci Methods
PubMed ID:17368788
'Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the ... More
CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission.
Authors:Jevsek M, Jaworski A, Polo-Parada L, Kim N, Fan J, Landmesser LT, Burden SJ
Journal:Proc Natl Acad Sci U S A
PubMed ID:16606832
'The genes encoding several synaptic proteins, including acetylcholine receptors, acetylcholinesterase, and the muscle-specific kinase, MuSK, are expressed selectively by a small number of myofiber nuclei positioned near the synaptic site. Genetic analysis of mutant mice suggests that additional genes, expressed selectively by synaptic nuclei, might encode muscle-derived retrograde signals that ... More
Constitutive sharing of recycling synaptic vesicles between presynaptic boutons.
Authors:Darcy KJ, Staras K, Collinson LM, Goda Y
Journal:Nat Neurosci
PubMed ID:16462738
'The synaptic vesicle cycle is vital for sustained neurotransmitter release. It has been assumed that functional synaptic vesicles are replenished autonomously at individual presynaptic terminals. Here we tested this assumption by using FM dyes in combination with fluorescence recovery after photobleaching and correlative light and electron microscopy in cultured rat ... More
Using FM1-43 to study neuropeptide granule dynamics and exocytosis.
Authors:Brumback AC, Lieber JL, Angleson JK, Betz WJ
Journal:Methods
PubMed ID:15183177
In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide ... More
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