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Citations & References (15)
Invitrogen™
HCS DNA Damage Kit
The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage isRead more
| Catalog Number | Quantity |
|---|---|
| H10292 | 1 kit |
Catalog number H10292
Price (EUR)
556,00
Each
Quantity:
1 kit
Price (EUR)
556,00
Each
The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser139) which is induced in response to double-strand break (DSB) formation. To detect cytotoxicity, the Image-iT® DEAD™ Green is an impermeant dye in healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeAlexa Fluor™ 555, Hoechst 33258
Format96-well plate
Quantity1 kit
Shipping ConditionDry Ice
ColorOrange, Blue, Green
For Use With (Equipment)High Content Analysis Instrument
Product LineMolecular Probes
Product TypeDNA Damage kit
Unit SizeEach
Contents & Storage
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.Store at ≤-20°C, desiccate and protect from light.
Frequently asked questions (FAQs)
What are the fluorescence excitation/emission maxima for the dyes contained in the HCS DNA Damage Kit?
Citations & References (15)
Citations & References
Abstract
Development of a high-content screening method for chemicals modulating DNA damage response.
Journal:J Biomol Screen
PubMed ID:21233308
The cellular response to DNA damage is emerging as a promising target for cancer therapy. In the present study, the authors exploited the relationship between the level of the phosphorylated form of histone H2AX (?H2AX) and the extent of DNA damage and developed a quantitative, cell-based, high-content screening system for
Induction of glutathione-dependent DNA double-strand breaks by the novel anticancer drug brostallicin.
Journal:Mol Cancer Ther
PubMed ID:19584235
Brostallicin is a DNA minor groove binder in phase II clinical trials. Here, we show that brostallicin induces gamma-H2AX nuclear foci that colocalize with 53BP1 and are dependent on glutathione, as shown by inhibition of those gamma-H2AX foci by l-buthionine sulfoximine. To differentiate brostallicin from the clinically approved minor groove
Free radical scavenging, a-glucosidase inhibitory and lipase inhibitory activities of eighteen Sudanese medicinal plants.
Journal:BMC Complement Altern Med
PubMed ID:30340582
'Lifestyle-related diseases such as diabetes are steadily increasing worldwide. In Sudan, there are a variety of plant species used traditionally for the treatment of diabetes, obesity and other symptoms which need to be validated through scientific studies for their claimed traditional uses. Therefore, in the current study, the free radical
Bioactive peptide amphiphile nanofiber gels enhance burn wound healing.
Journal:Burns
PubMed ID:31043333
'Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have
A novel hydroxyphenyl hydrazone derivate YCL0426 inhibits cancer cell proliferation through sequestering iron.
Journal:Anticancer Drugs
PubMed ID:28926421
'Cancer cells have an increased requirement for iron than normal cells, and iron chelators are under active consideration for cancer treatment. The metal-sequestering potential and antiproliferative mechanisms of a novel hydroxyphenyl hydrazone derivate YCL0426 were investigated here. Antiproliferative activity of YCL0426 was detected by MTT assay. The iron-sequestering potential was