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Thermo Scientific Chemicals

Laemmli SDS sample buffer, reducing (6X)

Catalog number: J61337.AD
50 mL, Each
Laemmli SDS sample buffer, reducing (6X)
Certificates
Thermo Scientific Chemicals

Laemmli SDS sample buffer, reducing (6X)

Catalog number: J61337.AD
50 mL, Each
Quantity
Certificates
Informational:Informational:This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.
Catalog number: J61337.AD
also known as J61337-AD
Price (EUR)
122,40 Online price
136,00
Each
Quantity
-
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Specifications

Specification SheetSpecification Sheet
Composition
375mM Tris-HCl/Tris Base, 9% SDS (w/v), 50% Glycerol (v/v), 9% 2-mercaptoethanol (v/v), 0.075% Bromophenol blue (w/v)
Appearance (Color)
Clear blue
Form
Liquid
pH
6.6-7.0 (1X)

Description

For protein sample preparation to be used in the Laemmli SDS-PAGE system. Laemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Further, it is used for polyacrylamide protein gel analysis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • This compound is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% β-mercaptoethanol, 0.03% bromophenol blue
  • The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it binds

Application

  • Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system
  • This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel
  • It is also used for polyacrylamide protein gel analysis
RUO – Research Use Only

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    Safety and Handling


    Classification of the substance or mixture
    CLP classification - Regulation(EC) No 1272/2008
    Acute oral toxicity
    Category 4
    Acute dermal toxicity
    Category 3
    Acute Inhalation Toxicity - Vapors
    Category 4
    Serious Eye Damage/Eye Irritation
    Category 1
    Skin Sensitization
    Category 1
    Skin Sensitization
    Sub-category 1A
    Chronic aquatic toxicity
    Category 2
    Label Elements
    Signal Word

    Danger

    Hazard Statements

    H302 + H332 - Harmful if swallowed or if inhaled

    H311 - Toxic in contact with skin

    H317 - May cause an allergic skin reaction

    H318 - Causes serious eye damage

    H411 - Toxic to aquatic life with long lasting effects

    Precautionary Statements

    P280 - Wear protective gloves/protective clothing/eye protection/face protection

    P301 + P330 + P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting

    P302 + P352 - IF ON SKIN: Wash with plenty of soap and water

    P304 + P340 - IF INHALED: Remove person to fresh air and keep comfortable for breathing

    P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

    P310 - Immediately call a POISON CENTER or doctor/physician