TE buffer, pH 8.0, RNAse free
TE buffer, pH 8.0, RNAse free
TE buffer, pH 8.0, RNAse free
TE buffer, pH 8.0, RNAse free
Thermo Scientific Chemicals

TE buffer, pH 8.0, RNAse free

CAS: 1185-53-1 | C4H12ClNO3 | 157.594 g/mol
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Quantity:
125 mL
250 mL
Catalog number J62745.EQE
also known as J62745-=E
Price (EUR)
107,00
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Quantity:
125 mL
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Price (EUR)
107,00
Each
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Specifications
CAS1185-53-1
Chemical Name or MaterialTE buffer
IUPAC Name2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI KeyQKNYBSVHEMOAJP-UHFFFAOYSA-N
MDL NumberMFCD00236359
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Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

Solubility
Not miscible or difficult to mix in water.

Notes
200mM Tris-HCl with 20mM EDTA
RUO – Research Use Only

General References:

  1. H Ito, et al. Transformation of intact yeast cells treated with alkali cations.J. Bacteriol.,1983,153,(1), 163-168.
  2. Hamilton O. Smith and Max L. Birnstiel. A simple method for DNA restriction site mapping. Nucl. Acids Res.,1976,3,(9), 2387-2398.