Agarose is useful in separation of nucleic acids electrophoretically, Suitable for isoelectric focusing, protein blotting and antibody separation Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Agarose is useful in separation of nucleic acids electrophoretically, Suitable for isoelectric focusing, protein blotting and antibody separation Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
Solubility
Soluble in water
Notes
Gel strength: >2400g/cm² (1.5% w/v); Gelling temperature: 35-37°C; EEO: 0.07-0.1
RUO – Research Use Only
General References:
- James M. Bailey.; Norman Davidson. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry. 1976, 70, (1), 75-85.
- Carl-Bertil Laurell. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry. 1966, 15, (1), 45-52.