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Citations & References (2)
Invitrogen™
InsectSelect™ Glow Kit with Sf9 Cells
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the followingRead more
| Catalog Number | Quantity |
|---|---|
| K81001 also known as K810-01 | 1 kit |
Catalog number K81001
also known as K810-01
Price (EUR)
3.010,00
Each
Quantity:
1 kit
Price (EUR)
3.010,00
Each
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSf9 Cells Glow Kit
Protein Tag Position (to your gene)C-terminal
Quantity1 kit
VectorpIZ
Cloning MethodRestriction Enzyme/MCS
Product LineInsectSelect
PromoterOplE2
Protein TagHis Tag (6x), V5 Epitope Tag
Unit SizeEach
Contents & Storage
The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Frequently asked questions (FAQs)
Does the incubator need to be humidified when culturing insect cells?
Citations & References (2)
Citations & References
Abstract
Disarming the mustard oil bomb.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12161563
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called
Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix.
Journal:J Biol Chem
PubMed ID:11805097
Punctin (ADAMTSL-1) is a secreted molecule resembling members of the ADAMTS family of proteases. Punctin lacks the pro-metalloprotease and the disintegrin-like domain typical of this family but contains other ADAMTS domains in precise order including four thrombospondin type I repeats. Punctin is the product of a distinct gene on human