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Citations & References (22)
Invitrogen™
Pacific Blue™ Succinimidyl Ester
Pacific Blue™ succinimidyl ester
| Catalog Number | Quantity |
|---|---|
| P10163 | 5 mg |
Catalog number P10163
Price (EUR)
542,00
Each
Quantity:
5 mg
Price (EUR)
542,00
Each
The amine-reactive Pacific Blue™ succinimidyl ester can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ∼410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser.
Related Links:
- View all Pacific Blue™ dye products..
- View the Fluorophore Selection Guide.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Detection MethodFluorescence
Emission451
Excitation416
Flow Cytometer Laser Lines405
FormatSolid
Label or DyePacific Blue™
Product TypeSuccinimidyl Ester
Quantity5 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
Label TypePacific Dyes
Product LinePacific Blue
Unit SizeEach
Contents & Storage
Contains 1 vial (1 mg). Store in freezer (-5 to -30°C) and protect from light.
Citations & References (22)
Citations & References
Abstract
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Journal:Cytometry A
PubMed ID:20824632
'Intracellular flow cytometry permits quantitation of diverse molecular targets at the single-cell level. However, limitations in detection sensitivity inherently restrict the method, sometimes resulting in the inability to measure proteins of very low abundance or to differentiate cells expressing subtly different protein concentrations. To improve these measurements, an enzymatic amplification
Reduction in muscle fibre number during the adaptive radiation of notothenioid fishes: a phylogenetic perspective.
Journal:J Exp Biol
PubMed ID:12819266
'The fish fauna of the continental shelf of the Southern Ocean is dominated by a single sub-order of Perciformes, the Notothenioidei, which have unusually large diameter skeletal muscle fibres. We tested the hypothesis that in fast myotomal muscle a high maximum fibre diameter (FD(max)) was related to a reduction in
Cytoskeletal-assisted dynamics of the mitochondrial reticulum in living cells.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12417764
'Subcellular organelle dynamics are strongly influenced by interactions with cytoskeletal filaments and their associated motor proteins, and lead to complex multiexponential relaxations that occur over a wide range of spatial and temporal scales. Here we report spatio-temporal measurements of the fluctuations of the mitochondrial reticulum in osteosarcoma cells by using
High-content single-cell drug screening with phosphospecific flow cytometry.
Journal:Nat Chem Biol
PubMed ID:18157122
'Drug screening is often limited to cell-free assays involving purified enzymes, but it is arguably best applied against systems that represent disease states or complex physiological cellular networks. Here, we describe a high-content, cell-based drug discovery platform based on phosphospecific flow cytometry, or phosphoflow, that enabled screening for inhibitors against
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling.
Journal:Nat Methods
PubMed ID:16628206
'Flow cytometry allows high-content, multiparameter analysis of single cells, making it a promising tool for drug discovery and profiling of intracellular signaling. To add high-throughput capacity to flow cytometry, we developed a cell-based multiplexing technique called fluorescent cell barcoding (FCB). In FCB, each sample is labeled with a different signature,