Sorbitol MacConkey with Cefixime Tellurite Agar/X.L.D. Agar Biplate

Efficiently isolate pathogenic Escherichia coli O157:H7 while simultaneously isolating and differentiating Salmonella and Shigella from clinical specimens with Thermo Scientific™ Oxoid™ Sorbitol MacConkey with Cefixime Tellurite Agar/X.L.D. Agar Biplate.

The Sorbitol MacConkey with Cefixime Tellurite Agar enables selective differentiation of Escherichia coli O157:H7 while X.L.D. Agar provides primary differentiation of Shigella and Salmonella species.

Produce more diagnostic details of pathogenic organisms while reducing storage capacity issue in the incubator with a bi-plate that combines Sorbitol MacConkey with Cefixime Tellurite Agar and X.L.D. Agar.

• Easy-to-read: easily differentiate Salmonella (red colonies with black centers) from Shigella (red colonies) and other coliforms (yellow, opaque colonies) with X.L.D. Agar and Escherichia coli O157: H7 (colorless colonies) with Sorbitol MacConkey with Cefixime Tellurite Agar.
• Ready-to-use: convenience of prepared media
• Space saving: bi-plate format

The formulation of Sorbitol MacConkey with Cefixime Tellurite Agar is identical to MacConkey Agar No.3, except that lactose has been replaced with sorbitol. E. coli O157: H7 does not ferment sorbitol and, therefore, produces colorless colonies. In contrast, most E. coli strains ferment sorbitol and form pink colonies. The addition of cefixime and tellurite significantly reduces the number of sorbitol non-fermenters that need to be screened during the attempted isolation of E. coli O157: H7.

X.L.D. Agar is a selective media for the isolation and differentiation of Salmonella, relying on xylose fermentation, lysine decarboxylation and production of hydrogen sulfide for the primary differentiation of Shigella and Salmonella spp. from non-pathogenic bacteria. The sensitivity and selectivity of XLD Agar exceeds that of traditional plating media, e.g., Eosin Methylene Blue and Bismuth Sulphite agars, which tend to suppress the growth of shigellae. The recovery of salmonellae and shigellae is not obscured by a profuse growth of other species; therefore, XLD Agar is ideal for the screening of samples containing mixed flora and suspected of harboring enteric pathogens, e.g., medical specimens or food products.

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