Bile Esculin Azide Agar

Isolate, enumerate and presumptively identify group D streptococci and enterococci using Thermo Scientific™ Bile Esculin Azide Agar, a microbiological media that includes a high portion of Ox bile.

Ox bile present in the media inhibits the growth of Gram-positive bacteria except Enterococci, while Sodium azide suppresses the Gram-negative bacteria. Group D streptococci and enterococci are able to hydrolyse aesculin into aesculetin and glucose, and the bondage of aesculetin and ferric ammonium citrate causes the brown-black to black halo around the colonies.

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