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Isolate and differentiate Shigella and Salmonella spp. from clinical specimens and food, with this selective medium.
Isolate and differentiate Shigella and Salmonella spp. from clinical specimens and food, with ready-to-use Thermo Scientific™ Oxoid™ Hektoen Enteric Agar. This selective medium is recommended for the isolation of salmonella from foods according to the Bacteriological Analytical Manual1. It is also recommended with the ISO 215672 standards for the isolation of shigellae from foods.
Hektoen Enteric Agar is a differential and selective medium for the isolation of Shigella and Salmonella spp.
The high peptone content in the medium offsets the inhibitory effects of bile salts on Shigella species in particular. The additional carbohydrates (sucrose and salicin) give better differentiation than lactose alone and the lower toxicity of the double indicator improves recovery. The increased lactose content helps early recognition of slow lactose-fermenting organisms. The thiosulphate and ferric citrate are present to detect H2S producing organisms.
1. US Food and Drug Administration. Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella May 2014. On line. AOAC International, Gaithersburg, Md.
2. ISO 21567:2004 Microbiology of food an animal feeding stuffs - Horizontal method for the detection of Shigella spp.