Streptavidin, Alexa Fluor™ 680 Conjugate
Streptavidin, Alexa Fluor™ 680 Conjugate
Citations & References (8)
Invitrogen™

Streptavidin, Alexa Fluor™ 680 Conjugate

Alexa Fluor™ 680 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin hasRead more
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Catalog NumberQuantityForm
S213781 mgSolid
S323580.5 mLLiquid
Catalog number S21378
Price (EUR)
442,00
Each
Add to cart
Quantity:
1 mg
Form:
Solid
Price (EUR)
442,00
Each
Add to cart
Alexa Fluor™ 680 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 680 Streptavidin Conjugates:
• Alexa Fluor™ 680 streptavidin conjugate has Ex/Em maxima of ∼ (679/702)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor™ Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label or DyeAlexa Fluor Dyes
Product TypeStreptavidin Conjugate (fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 680
FormSolid
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (8)

Citations & References
Abstract
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Authors:Nikiforov TT, Beechem JM
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More
Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs.
Authors:Veerman KM, Williams MJ, Uchimura K, Singer MS, Merzaban JS, Naus S, Carlow DA, Owen P, Rivera-Nieves J, Rosen SD, Ziltener HJ
Journal:Nat Immunol
PubMed ID:17401367
'P-selectin glycoprotein ligand 1 (PSGL-1) is central to the trafficking of immune effector cells to areas of inflammation through direct interactions with P-selectin, E-selectin and L-selectin. Here we show that PSGL-1 was also required for efficient homing of resting T cells to secondary lymphoid organs but functioned independently of selectin ... More
Reversibility of covalent electrophile-protein adducts and chemical toxicity.
Authors:Lin D, Saleh S, Liebler DC,
Journal:Chem Res Toxicol
PubMed ID:19548357
The biotin-tagged electrophiles 1-biotinamido-4-(4'-[maleimidoethylcyclohexane]-carboxamido)butane (BMCC) and N-iodoacetyl-N-biotinylhexylenediamine (IAB) have been used as model electrophile probes in complex proteomes to identify protein targets associated with chemical toxicity. Whereas IAB activates stress signaling and apoptosis in HEK293 cells, BMCC does not. Cysteine Michael adducts formed from BMCC and nonbiotinylated analogues rapidly disappeared ... More
Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.
Authors:Bitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
Journal:Hum Mutat
PubMed ID:19623537
Dynamin 2 (DNM2) is a large GTPase involved in the release of nascent vesicles during endocytosis and intracellular membrane trafficking. Distinct DNM2 mutations, affecting the middle domain (MD) and the Pleckstrin homology domain (PH), have been identified in autosomal dominant centronuclear myopathy (CNM) and in the intermediate and axonal forms ... More
Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors.
Authors:van Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB
Journal:Nat Struct Mol Biol
PubMed ID:17187076
The ability of pathogenic bacteria to recognize host glycans is often essential to their virulence. Here we report structure-function studies of previously uncharacterized glycogen-binding modules in the surface-anchored pullulanases from Streptococcus pneumoniae (SpuA) and Streptococcus pyogenes (PulA). Multivalent binding to glycogen leads to a strong interaction with alveolar type II ... More
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