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Citations & References (11)
Invitrogen™
Vybrant™ Alexa Fluor™ 594 Lipid Raft Labeling Kit
Vybrant Lipid Raft Labeling Kits are designed to provide convenient, reliable and extremely bright fluorescent labeling of lipid rafts inRead more
| Catalog Number | Quantity |
|---|---|
| V34405 | 1 kit |
Catalog number V34405
Price (EUR)
1.186,00
1 kit
Quantity:
1 kit
Price (EUR)
1.186,00
1 kit
Vybrant Lipid Raft Labeling Kits are designed to provide convenient, reliable and extremely bright fluorescent labeling of lipid rafts in live cells. Kits include cholera toxin subunit B (CT-B) labeled with either the green-fluorescent Alexa Fluor 488, the orange-fluorescent Alexa Fluor 555 or the red-fluorescent Alexa Fluor 594 dye, as well as an antibody that specifically recognizes CT-B. A 10X solution of phosphate-buffered saline (10X PBS), pH 7.2, and detailed instructions are also included in the kits.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineAlexa Fluor
Protein SubtypeCholera Toxin
Quantity1 kit
Shipping ConditionWet Ice
Label TypeAlexa Fluor Dyes
Product TypeLipid Raft Labeling Kit
SubCellular LocalizationCell Membranes and Lipids
Unit Size1 kit
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (11)
Citations & References
Abstract
Looking at lipid rafts?
Journal:Trends Cell Biol
PubMed ID:10201072
'The notion that microdomains enriched in certain specialized lipids exist in membranes has been both attractive and controversial since it was first proposed that such domains, termed rafts, might act as apical sorting devices in epithelial cells. The observation that certain lipids are not extractable in cold nonionic detergent supports
Functions of lipid rafts in biological membranes.
Journal:Annu Rev Cell Dev Biol
PubMed ID:9891780
'Recent studies showing that detergent-resistant membrane fragments can be isolated from cells suggest that biological membranes are not always in a liquid-crystalline phase. Instead, sphingolipid and cholesterol-rich membranes such as plasma membranes appear to exist, at least partially, in the liquid-ordered phase or a phase with similar properties. Sphingolipid and
Lipid rafts make for slippery platforms.
Journal:J Cell Biol
PubMed ID:12885764
What's in a raft? Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial. The answers will shape our views of signaling and of membrane dynamics.
Lipid rafts and little caves. Compartmentalized signalling in membrane microdomains.
Journal:Eur J Biochem
PubMed ID:11846775
Lipid rafts are liquid-ordered membrane microdomains with a unique protein and lipid composition found on the plasma membrane of most, if not all, mammalian cells. A large number of signalling molecules are concentrated within rafts, which have been proposed to function as signalling centres capable of facilitating efficient and specific
Elevated NADPH oxidase activity contributes to oxidative stress and cell death in Huntington's disease.
Journal:Hum Mol Genet
PubMed ID:23223017
A mutation in the huntingtin (Htt) gene produces mutant Htt and Huntington's disease (HD), a neurodegenerative disorder. HD patients have oxidative damage in the brain, but the causes are unclear. Compared with controls, we found brain levels of NADPH oxidase (NOX) activity, which produces reactive oxygen species (ROS), elevated in