Pharma ProPac™ SAX-10 HPLC Columns

The Thermo Scientific Pharma ProPac SAX-10 HPLC Columns are for use in the separation of protein variants with exceptionally high resolution and efficiency. Based on a nonporous 10-µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Features of the Pharma ProPac SAX-10 HPLC Columns include:
• Ideal for characterization and quality control assessment of different AAV serotypes
• Unequaled high-resolution separations
• High-efficiency peaks with excellent recovery
• Higher capacity than other columns based on non-porous particles
• Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

Superior performance for AAV empty-to-full capsid ratio
Our columns assist chromatographers with viral vector analysis. Adeno-associated virus (AAV) is the vector of choice for many gene therapies. The AAV empty-to-full capsid ratio is a critical quality attribute that requires monitoring throughout the development cycle.

With a 20 year legacy of ion-exchange experience, the ProPac series of columns continues to assist chromatographers in the biotherapeutic space. Easily resolving empty-to-full capsid ratios of AAV6 and AAV8 serotypes in under 10 minutes, ProPac SAX-10 columns provide chromatographers nearly instant quantitative results, with minimal sample preparation.