DNAPac™ RP Columns

Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.

The DNAPac RP column is based on spherical wide-pore 4μm polymer resin. The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments. DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over.

• Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids
• High resolution and high throughput
• Excellent MS compatibility
• Wide operating pH range (0-14) and high temperature stability (up to 110°C)