Gateway™ pEF-DEST51 Vector

Citations & References (1)

Invitrogen™

Gateway™ pEF-DEST51 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,Read more

Catalog Number	Quantity
12285011 also known as 12285-011	6 μg

Catalog number 12285011

also known as 12285-011

Price (EUR)

1.418,00

Add to cart

Quantity:

Price (EUR)

1.418,00

Add to cart

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible SystemConstitutive

Delivery TypeTransfection

For Use With (Application)Constitutive Expression

Product TypeGateway System Destination Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)Blasticidin

VectorpDEST

Cloning MethodGateway

Product LineGateway

PromoterEF-1α

Protein TagHis Tag (6x), V5 Epitope Tag

Unit SizeEach

Contents & Storage

All destination vectors are provided lyophilized and supercoiled.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pEF-DEST51 Vector FAQs

Citations & References (1)

Citations & References

Abstract

The FAM deubiquitylating enzyme localizes to multiple points of protein trafficking in epithelia, where it associates with E-cadherin and beta-catenin.

Authors:Murray RZ, Jolly LA, Wood SA,

Journal:Mol Biol Cell

PubMed ID:14742711

'Ubiquitylation is a necessary step in the endocytosis and lysosomal trafficking of many plasma membrane proteins and can also influence protein trafficking in the biosynthetic pathway. Although a molecular understanding of ubiquitylation in these processes is beginning to emerge, very little is known about the role deubiquitylation may play. Fat ... More