Amphotericin B
Amphotericin B
Citations & References (5)
Gibco™

Amphotericin B

Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin BRead more
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Catalog NumberQuantity
1529002650 mL
1529001820 mL
Catalog number 15290026
Price (EUR)
35,51
Each
Add to cart
Quantity:
50 mL
Price (EUR)
35,51
Each
Add to cart
Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 μg of amphotericin B and 205 μg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 μg/mL.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.25 to 2.5 μg/mL
For Use With (Application)Prevention of Cell Culture Contamination
Product LineFungizone
Quantity50 mL
Shelf Life12 Months
Shipping ConditionDry Ice
FormLiquid
Product TypeAntifungal
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How many freeze/thaw cycles do you recommend for Amphotericin B?

Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of Amphotericin B when stored at 4 degrees C?

Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Fungizone and Amphotericin B?

Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (5)

Citations & References
Abstract
PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.
Authors:Wiatrak B, Kubis-Kubiak A, Piwowar A, Barg E
Journal:Cells
PubMed ID:32295099
'The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend ... More
Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.
Authors:Qian X, Jacob F, Song MM, Nguyen HN, Song H, Ming GL
Journal:Nat Protoc
PubMed ID:29470464
Human brain organoids, 3D self-assembled neural tissues derived from pluripotent stem cells, are important tools for studying human brain development and related disorders. Suspension cultures maintained by spinning bioreactors allow for the growth of large organoids despite the lack of vasculature, but commercially available spinning bioreactors are bulky in size ... More
Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides.
Authors:Arrigo AP
Journal:Methods Mol Biol
PubMed ID:29177658
Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including aA,B-crystallin polypeptides, is characterized by a conserved C-terminal a-crystallin domain and molecular weights ranging from 20 to 40 kDa. Here, procedures are described for analyzing the dynamic oligomerization ... More
A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection.
Authors:Slon-Campos JL, Dejnirattisai W, Jagger BW, López-Camacho C, Wongwiwat W, Durnell LA, Winkler ES, Chen RE, Reyes-Sandoval A, Rey FA, Diamond MS, Mongkolsapaya J, Screaton GR
Journal:Nat Immunol
PubMed ID:31477918
Infections with dengue virus (DENV) and Zika virus (ZIKV) can induce cross-reactive antibody responses. Two immunodominant epitopes-one to precursor membrane protein and one to the fusion loop epitope on envelope (E) protein-are recognized by cross-reactive antibodies ... More
Establishment and Genetic Landscape of Precancer Cell Model Systems from the Head and Neck Mucosal Lining.
Authors:de Boer DV, Brink A, Buijze M, Stigter-van Walsum M, Hunter KD, Ylstra B, Bloemena E, Leemans CR, Brakenhoff RH
Journal:Mol Cancer Res
PubMed ID:30224542
Head and neck squamous cell carcinomas (HNSCC) develop in fields of genetically altered cells. These fields are often dysplastic and a subset can be recognized as (erythro)leukoplakia, but most are macroscopically invisible. There is a lack of adequate treatment options to eradicate these fields, whereas they underlie the development of ... More