BLOCK-iT™ Fluorescent Oligo, for lipid transfection
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Invitrogen™

BLOCK-iT™ Fluorescent Oligo, for lipid transfection

BLOCK-iT™ Fluorescent Oligo provides:• Strong, easily detectable FITC signal indicating transfection efficiency• Chemical modifications for a clearer, more persistent cellularRead more
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Catalog NumberQuantity
20132 x 125 μL
Catalog number 2013
Price (EUR)
466,00
Each
Add to cart
Quantity:
2 x 125 μL
Price (EUR)
466,00
Each
Add to cart
BLOCK-iT™ Fluorescent Oligo provides:

• Strong, easily detectable FITC signal indicating transfection efficiency
Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA
Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes the BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. BLOCK-iT™ Fluorescent Oligo is available in two configurations: one designed for lipid-mediated transfection (2 x 125 μL) at 20 μM and one designed for electroporation (75 μl) at 1 mM.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Lipid Transfection
Label or DyeFITC (Fluorescein)
Product TypeOligonucleotides
Quantity2 x 125 μL
Product LineBLOCK-iT
Unit SizeEach
Contents & Storage
The BLOCK-iT™ Fluorescent Oligo for lipid transfection is supplied as 2 x 125 μl tubes at 20 μM. Stored at -20°C.

Frequently asked questions (FAQs)

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?

  • We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
  • BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.


  • Find additional tips, troubleshooting help, and resources within our RNAi Support Center

    Citations & References (4)

    Citations & References
    Abstract
    RNAi specificity: how big of an issue is it?
    Authors:Samarsky DA, Welch PJ,
    Journal:Pharmacogenomics
    PubMed ID:15723598
    RNAi techniques hold great promise for science, from basic research all the way to human therapeutics, and our understanding of the underlying mechanisms have allowed us to design better and more effective compounds. Fortunately, much has also been learned about nonspecific effects, such as off-target and stress responses. These observations and discoveries have led us to improved specificity ... More
    CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.
    Authors:Zhou S, Zhou H, Walian PJ, Jap BK,
    Journal:Proc Natl Acad Sci U S A
    PubMed ID:15890777
    'gamma-Secretase is a membrane protein complex that cleaves the beta-amyloid precursor protein (APP) within the transmembrane region, after prior processing by beta-secretase, producing amyloid beta-peptides Abeta(40) and Abeta(42). Errant production of Abeta-peptides that substantially increases Abeta(42) production has been associated with the formation of amyloid plaques in Alzheimer''s disease patients. ... More
    Differentiation of mouse embryonic stem cells following RNAi-mediated silencing of OCT4 and Nanog.
    Authors:Hough SR, Clements I, Welch PJ, Wiederholt KA,
    Journal:Stem Cells
    PubMed ID:16456133
    RNAi holds great promise as a tool to study the basic biology of stem cells or to direct differentiation in a specific manner. Barriers to achieving efficient and specific gene silencing in RNAi experiments include limitations in transfection efficiency and in the efficacy and specificity of RNAi silencing effectors. Here, ... More
    IpgB1 is a novel Shigella effector protein involved in bacterial invasion of host cells. Its activity to promote membrane ruffling via Rac1 and Cdc42 activation.
    Authors:Ohya K, Handa Y, Ogawa M, Suzuki M, Sasakawa C,
    Journal:J Biol Chem
    PubMed ID:15849186
    Shigella, the causative agent of bacillary dysentery, is capable of inducing the large scale membrane ruffling required for the bacterial invasion of host cells. Shigella secrete a subset of effectors via the type III secretion system (TTSS) into the host cells to induce membrane ruffling. Here, we show that IpgB1 ... More