PageRuler™ Unstained High Range Protein Ladder
PageRuler™ Unstained High Range Protein Ladder
Thermo Scientific™

PageRuler™ Unstained High Range Protein Ladder

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) forRead more
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Catalog number 26637
Price (EUR)/ 2 x 250 µL
176,00
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Price (EUR)/ 2 x 250 µL
176,00
Add to cart
PageRuler™ Unstained High Range Protein Ladder
Catalog number26637
Price (EUR)/ 2 x 250 µL
176,00
-
Add to cart
Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained High Range Protein Ladder:

Size range—8 proteins spanning 60 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—150kDa band has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Number of Markers8
Product TypeProtein Ladder
Ready to LoadYes
Recommended ApplicationsTris-acetate gels
Size Range60 to 250 kDa
Stain TypeUnstained
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)250, 200, 150, 120, 100, 85, 70, 60 kDa
Product LinePageRuler™
Quantity2 x 250 mL
Shipping ConditionApproved for shipment on Wet or Dry Ice
System TypeWestern Blotting, SDS-PAGE
Unit Size2 x 250 µL
Contents & Storage
Upon receipt store at -20°C. Product is shipped with an ice pack.