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Citations & References (15)
Thermo Scientific™
EZ-Link™ Plus Activated Peroxidase Kit
Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greaterRead more
| Catalog Number | Quantity |
|---|---|
| 31489 | 5 reactions |
Catalog number 31489
Price (EUR)
472,00
Each
Quantity:
5 reactions
Price (EUR)
472,00
Each
Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins. Each kit contains 5 x 1 mg of lyophilized HRP and sufficient Sodium cyanoborohydride solution, quenching buffer, and buffers to conjugate 5 mg of Immunoglobulin (IgG)
Features of the Thermo Scientific Pierce Plus Activated Peroxidase Kit:
• Activated HRP – periodate-treated, aldehyde-activated horseradish peroxidase, ready for conjugation to antibodies and other proteins at sites of primary amines (e.g., lysines)
• Permanent conjugation – reacts efficiently (95%) with primary amines to form covalent amide bonds upon treatment with sodium cyanoborohydride (included in kit)
• High activity HRP – enzyme activity is 120 to 200 units/mg; lyophilized, activated enzyme is stable for at least 12 months at -20°C
• Convenient quantities – each 1 mg-quantity of activated enzyme is sufficient for reaction with 1 mg of IgG to produce about 0.5 mL of conjugate
• Customizable – vary the molar ratios, reaction buffer and pH, and other parameters to acheive conjugates with different levels of HRP incorporation and activity
Plus Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Plus Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.
Related Products
EZ-Link™ Plus Activated Peroxidase
Features of the Thermo Scientific Pierce Plus Activated Peroxidase Kit:
• Activated HRP – periodate-treated, aldehyde-activated horseradish peroxidase, ready for conjugation to antibodies and other proteins at sites of primary amines (e.g., lysines)
• Permanent conjugation – reacts efficiently (95%) with primary amines to form covalent amide bonds upon treatment with sodium cyanoborohydride (included in kit)
• High activity HRP – enzyme activity is 120 to 200 units/mg; lyophilized, activated enzyme is stable for at least 12 months at -20°C
• Convenient quantities – each 1 mg-quantity of activated enzyme is sufficient for reaction with 1 mg of IgG to produce about 0.5 mL of conjugate
• Customizable – vary the molar ratios, reaction buffer and pH, and other parameters to acheive conjugates with different levels of HRP incorporation and activity
Plus Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Plus Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.
Related Products
EZ-Link™ Plus Activated Peroxidase
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Labeling Scale0.5-1 mg
Labeling TargetAntibodies (General), Proteins (General)
Label or DyeHRP (Horseradish Peroxidase)
Quantity5 reactions
Sufficient For5 Enzyme Labeling Reactions
Product LineEZ-Link
Unit SizeEach
Contents & Storage
Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)
• Plus Activated Peroxidase, 5 x 1 mg
• Sodium cyanoborohydride solution, 1 x 0.5 mL
• Quenching Buffer, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack
Store at -20°C.
• Plus Activated Peroxidase, 5 x 1 mg
• Sodium cyanoborohydride solution, 1 x 0.5 mL
• Quenching Buffer, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack
Store at -20°C.
Citations & References (15)
Citations & References
Abstract
Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays.
Journal:
PubMed ID:26544909
Single domain heavychain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. However, there is no simple method to select the most valuable nanobodies from the crowd of positive clones obtained after the initial screening. In this paper, we describe a novel nanobody-based platform that
Generation and characterization of antibodies against Asian elephant (Elephas maximus) IgG, IgM, and IgA.
Journal:
PubMed ID:25658336
Asian elephant (Elephas maximus) immunity is poorly characterized and understood. This gap in knowledge is particularly concerning as Asian elephants are an endangered species threatened by a newly discovered herpesvirus known as elephant endotheliotropic herpesvirus (EEHV), which is the leading cause of death for captive Asian elephants born after 1980
A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post-vaccination monitoring.
Journal:BMC Vet Res
PubMed ID:31937302
'Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention.
A novel monoclonal antibodies-based sandwich ELISA for detection of serotype 4 fowl adenovirus.
Journal:Avian Pathol
PubMed ID:30621493
'As a major causative agent for hepatitis-hydropericardium syndrome (HPS) in chickens, serotype 4 fowl adenovirus (FAdV-4) has caused huge economic losses in the poultry industry globally. However, there is no commercial diagnostic test for FAdV-4 antigens. To generate a rapid approach for specific detection of FAdV-4, a monoclonal antibodies (mAbs)-based
A novel PSMA/GCPII-deficient mouse model shows enlarged seminal vesicles upon aging.
Journal:Prostate
PubMed ID:30256431
'Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is an important diagnostic and therapeutic target in prostate cancer. PSMA/GCPII is also expressed in many healthy tissues, but its function has only been established in the brain and small intestine. Several research groups have attempted to produce PSMA/GCPII-deficient