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Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular
Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids. | CAS: 136112-00-0 | C6H6O
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Price (EUR)
312,00
Each
Chemical Identifiers
CAS136112-00-0
IUPAC Name3-methylbutan-1-ol; phenol; trichloromethane
Molecular FormulaC12H19Cl3O2
InChI KeyZYWFEOZQIUMEGL-UHFFFAOYSA-N
SMILESClC(Cl)Cl.CC(C)CCO.OC1=CC=CC=C1
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Specifications
Specification Sheet
Specification SheetAppearance (Color)Clear yellow to orange
Appearance (Form)Solution
Heavy metals (as Pb)=<1 ppm
pH7.8 to 8.2 (at 25°C)
Iron (Fe)=<1 ppm
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This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
- Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
- When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
- It is also used to generate DNA minisatellite fingerprints
RUO – Research Use Only