IMDM, powder
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Gibco™

IMDM, powder

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.Read more
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Catalog NumberQuantity
42200014
also known as 42200-014
1 L
42200030
also known as 42200-030
10 L
Catalog number 42200014
also known as 42200-014
Price (EUR)
25,90
Each
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Quantity:
1 L
Customize this product
Price (EUR)
25,90
Each
Add to cart

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of Gibco™ IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This IMDM is modified as follows:
WithWithout
• L-glutamine• α-thioglycerol
• Phenol Red• 2-mercaptoethanol


The complete formulation is available.

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.

IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications
Cell TypeMammalian Cells
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality215 - 245 mOsm/kg
Product LineGibco
Product TypeIscove's Modified Dulbecco's Medium (IMDM)
Quantity1 L
Shelf Life36 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormPowder
With AdditivesGlutamine, HEPES, Phenol Red, Sodium Pyruvate
Without AdditivesNo Sodium Bicarbonate
pH4.4 to 4.8
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.

Frequently asked questions (FAQs)

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.