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| Catalog Number | Quantity |
|---|---|
| 42200014 also known as 42200-014 | 1 L |
| 42200030 also known as 42200-030 | 10 L |
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of Gibco™ IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
| With | Without |
| • L-glutamine | • α-thioglycerol |
| • Phenol Red | • 2-mercaptoethanol |
The complete formulation is available.
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.
IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).