AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2
AmpliTaq Gold&trade; DNA Polymerase with Gold Buffer and MgCl<sub>2</sub>
Citations & References (2)
Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
431774225,000 Units
4311806250 Units
43118161000 Units
43118203000 Units
43118185000 Units
Catalog number 4317742
Price (EUR)
18.790,00
Each
Add to cart
Quantity:
25,000 Units
Request bulk or custom format
Price (EUR)
18.790,00
Each
Add to cart
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Features

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Gold Buffer with MgCl2

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.

GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

Notes

  • AmpliTaq Gold 360 DNA Polymerase is available for even better PCR performance.
  • AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions20,000 Reactions
Overhang3'-A
PolymeraseAmpliTaq Gold DNA Polymerase
Product TypeDNA Polymerase
Quantity25,000 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
Concentration5 U/μL
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
This package includes 5 boxes, each box contains:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL
• GeneAmp 10X Gold Buffer, 20 x 1.5 mL
• 25 mM MgCl2, 20 x 1.5 mL

Store at -15°C to -30°C.

Frequently asked questions (FAQs)

What is the difference between AmpliTaq Gold polymerase and Platinum Taq polymerase?

Both AmpliTaq Gold and Platinum Taq are hot-start enzymes that allow you to set up your reactions on the benchtop without the need for ice. AmpliTaq Gold is a chemically-modified hot-start enzyme, provided in an inactive state. Heat activates the enzyme, with full activity after 10 min at 95 degrees C. Platinum Taq is an antibody-mediated hot-start enzyme. The anti-Taq antibodies bind and inactivate the enzyme, until the heat denaturation step of the PCR reaction (30 sec to 2 min), which activates the enzyme.

When using AmpliTaq Gold DNA polymerase, under what conditions would one choose a two-temperature vs. three-temperature PCR?

A two-temperature PCR is commonly used when the primer annealing temperatures are above 60 degrees C. Use a three-temperature PCR when the templates have high G+C content and/or secondary structure, or desired primer annealing temperatures are below 60 degrees C. Please consult the Product Insert for more information on the use of AmpliTaq Gold DNA polymerase.

Is there anything to prevent AmpliTaq Gold DNA polymerase from extending from the 3’ end of a TaqMan probe in a 5’ nuclease assay?

Yes. There is a phosphate group on the 3' end of all TaqMan probes that prevents such extension.

How does AmpliTaq Gold DNA Polymerase differ from AmpliTaq DNA Polymerase?

AmpliTaq Gold DNA Polymerase is a modified form of AmpliTaq DNA Polymerase that contains a proprietary chemical (or so-called hot start molecule) bound to the enzyme's active site. In order to activate the AmpliTaq Gold DNA Polymerase fully, we recommend an initial activation step of 95 degrees C for 10 min when using GeneAmp 10X PCR Buffer I and/or GeneAmp 10X PCR Buffer II and Mg in one of our thermal cyclers. When using GeneAmp 10X PCR Gold Buffer, activation time can be reduced to 5 minutes. Once activation is complete, you can proceed with your standard PCR cycling program (denaturing, annealing, extension, etc).

Does AmpliTaq Gold DNA Polymerase contain exonuclease (proofreading) activity?

No, AmpliTaq Gold DNA polymerase does not contain proofreading activity, however fidelity in PCR amplifications utilizing this enzyme may be improved. High fidelity can be achieved by: 1. Decreasing the final concentration of each nucleotide to 40-50 uM. 2. Using the lowest MgCl2 concentration possible. 3. Using less enzyme. 4. Decreasing extension times. 5. Using the highest annealing temperature possible. 6. Using as few cycles as possible.

View all AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 FAQs

Citations & References (2)

Citations & References
Abstract
Approaches to determine expression of inflammatory cytokines.
Authors:Amsen D, de Visser KE, Town T,
Journal:Methods Mol Biol
PubMed ID:19347295
'There is an increasing awareness of the role of inflammation in cancer. Immune responses can limit the growth of some tumors, but paradoxically, may promote the growth of others. Cytokines are critical mediators of both the innate and the adaptive immune responses. In this chapter, we will describe several methods ... More
A phase 1 study of a vaccine targeting preferentially expressed antigen in melanoma and prostate-specific membrane antigen in patients with advanced solid tumors.
Authors:Weber JS, Vogelzang NJ, Ernstoff MS, Goodman OB, Cranmer LD, Marshall JL, Miles S, Rosario D, Diamond DC, Qiu Z, Obrocea M, Bot A,
Journal:J Immunother
PubMed ID:21760528
Preferentially expressed antigen in melanoma (PRAME) and prostate-specific membrane antigen (PSMA) are tumor-associated antigens implicated in cellular differentiation, genetic stability, and angiogenesis. MKC1106-PP is an immunotherapeutic regimen cotargeting PRAME and PSMA, comprised of a recombinant plasmid (pPRA-PSM encoding fragments derived from both antigens) and 2 peptides (E-PRA and E-PSM derived ... More