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Custom TaqMan™ Gene Expression Assay, VIC
Applied Biosystems™

Custom TaqMan™ Gene Expression Assay, VIC

Applied Biosystems Custom TaqMan Gene Expression assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.
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Catalog NumberQuantity
4448509M (750 reactions), made to order
4448508S (360 reactions), made to order
4448510L (2900 reactions), made to order
Catalog number 4448509
Price (EUR)
600,00
Each
Quantity:
M (750 reactions), made to order

Applied Biosystems Custom TaqMan Gene Expression assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. Custom TaqMan Gene Expression assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Features include:

  • Easy to use – just add cDNA and master mix and run the qPCR—no melt curves required
  • Specific – TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive – TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
  • Accurate – identify small fold-changes with high accuracy of quantitation
  • Gold-standard TaqMan qPCR chemistry – TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
  • Ideal for multiplexing – combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
  • Sequences provided – with every Custom TaqMan Gene Expression you design and order

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay

  • 2 unlabeled PCR primers (900 nM each final 1X concentration)
  • 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Specifications
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
No. of Reactions750 reactions
PrimerLabeled PCR
Product LineTaqMan
Product TypeGene Expression Assay
QuantityM (750 reactions), made to order
Shipping ConditionRoom Temperature
Concentration20X
Unit SizeEach
Contents & Storage
1 Tube containing a 20 x (S and M sizes) or 60 x (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store (at -15°C to -25°C.

)

Frequently asked questions (FAQs)

How do I determine if Applied Biosystems TaqMan Assays that I want to multiplex are compatible with each other?

You can utilize the Applied Biosystems TaqMan Multiplex Interaction Check (MIC) tool. The MIC tool uses bioinformatics to identify potential interactions between primers, probes, and amplicons that could negatively impact multiplex qPCR performance. The tool was developed and validated using data from approximately 200 multiplex panels, combining computational analysis with wet-lab testing. By flagging assay combinations that may be prone to false positives or reduced sensitivity, MIC tool helps researchers design multiplex panels with greater confidence and reduce time spent on trial-and-error optimization.risk and observed multiplex performance.

Why are the concentrations of forward and reverse primer in our Primer-Limited (PL) Applied Biosystems TaqMan Gene Expression Assays?

Primer-Limited Applied Biosystems TaqMan Gene Expression Assays with FAM, ABY, JUN, or Cyanine 5 dyes have a primer concentration of 350 nM. Assays with VIC dyes have a primer concentration of 150 nM.

Why are some Applied Biosystems TaqMan Gene Expression Assays only available in Primer-Limited concentrations?

In a multiplex qPCR reaction, like in Applied Biosystems TaqMan Gene Expression Assays, targets often differ in abundance and amplification efficiency. Highly expressed or highly efficient targets can consume a disproportionate share of reaction components, generate very strong fluorescent signals, and outcompete weaker targets for polymerase and other reagents. Primer-limited assays provide balanced amplification of targets in multiplex workflows by reducing primer competition between assays and supporting more consistent amplification across multiple targets.

How many Applied Biosystems TaqMan Gene Expression Assays can I multiplex in a single reaction?

You can multiplex up to five Applied Biosystems TaqMan Gene Expression Assays utilizing FAM, VIC, ABY, JUN, and Cyanine 5 dyes.

Can I check the end product of a TaqMan Gene Expression Assay by running an agarose gel?

Yes, you can check the end product on an agarose gel. If you use the TaqMan Universal PCR Master Mix (including AmpErase UNG, Cat. No. 4304437), we recommend running the gel immediately after the PCR, since the trace amount of AmpErase UNG may digest the end product that contains double-stranded DNA with dUTP incorporated. The average length of amplicon ranges from 50-150 bp. The exact amplicon length is indicated on the detailed page of each assay on our website. You can get to that page by clicking on the Assay ID; the amplicon information is listed in the section "Assay Details".

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