Ion Chef™ Instrument

The Ion Chef™ system automates Ion AmpliSeq™ library preparation, template preparation, and chip loading for targeted next-generation sequencing (NGS) workflows on the Ion GeneStudio™ S5 systems. Cartridge-based reagents, integrated quality checks, and an intuitive touchscreen interface simplify sample preparation and reduce hands-on time. The standardized workflow helps laboratories generate consistent sequencing-ready chips with fewer manual processing steps.

Key features

• Automated Ion AmpliSeq library preparation, template preparation, and chip loading
• Cartridge-based reagent system with integrated sample and reagent tracking
• Less than 15 minutes of hands-on setup each for library preparation and template preparation workflows
• Standardized workflows that help reduce user-to-user variability

Simplify the Ion Torrent™ NGS workflow

The Ion Chef system combines library preparation, template preparation, and chip loading into a single automated workflow. Library construction, equalization, pooling, template preparation, and chip loading are performed using preconfigured protocols and consumables. This connected workflow reduces manual processing steps and provides a straightforward approach to prepare samples for sequencing.

Consistent automated sample preparation

Automated barcode identification, reagent verification, onboard diagnostics, and workflow checks support consistent instrument setup and operation. Standardized processing helps reduce variability associated with manual sample preparation and provides reproducible chip loading across supported workflows.

Save time and labor

Typical setup for library preparation and template preparation requires less than 15 minutes of hands-on time for each workflow. Automation allows laboratory personnel to spend less time performing repetitive sample preparation tasks and more time on sequencing projects, data review, and other laboratory activities.

Flexible support for targeted sequencing

The Ion Chef system supports a broad range of targeted sequencing applications using Ion AmpliSeq panels and workflows. Laboratories can perform automated library preparation for oncology, inherited disease, reproductive health, infectious disease, microbiology, transcriptomics, and custom targeted sequencing applications using a common workflow across the Ion GeneStudio S5 systems.

Services and support

Thermo Fisher Scientific offers service and support programs for the Ion Chef system to help maintain instrument performance throughout its lifecycle.

• Service plans:—Instrument service agreements designed to support routine operation and system availability.
• Qualification services:—Installation and operational qualification services that document instrument performance according to manufacturer specifications.
• Training:—Instructor-led and virtual courses covering instrument operation, workflow setup, troubleshooting, and NGS applications.
• Bioinformatics support:—Resources for workflow implementation, software configuration, data analysis, and downstream applications.