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Thermo Scientific™
Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
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| Catalog Number | Quantity |
|---|---|
| 78426 | 5 x 1 mL |
| 78420 | 1 mL |
| 78428 | 24 x 100 μL |
| 78427 | 10 mL |
Catalog number 78426
Price (EUR)
742,00
Each
Quantity:
5 x 1 mL
Price (EUR)
742,00
Each
Thermo Scientific Halt Phosphatase Inhibitor Single Use Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.
Features of Halt Phosphatase Inhibitor Single Use Cocktail:
- Devoid of protease inhibitors—To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (Cat. No. 78420)
- Dialyzable— Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
- Supplied as a 100X concentrate—effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
- Compatibility— compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
- All-in-one format—inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
- Compatible with popular protein assays—works with standard protein assays, including BCA and coomassie (Bradford) assays
- Convenient storage—refrigerator (2 to 8°C) storage saves valuable space in the freezer and provides for immediate use
Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays.
Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.
Related Products
- Pierce™ Phosphatase Inhibitor Mini Tablets (Cat. No. 88667)
For Research Use Only. Not for use in diagnostic procedures.
Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways with the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.
The Halt Phosphatase Inhibitor Cocktail does not contain protease inhibitors. If necessary, protease inhibitors can be added along with the phosphatase inhibitor cocktail. Halt Phosphatase Inhibitor Cocktail is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Where incompatibilities arise, the components can be removed by dialysis or desalting before performing these incompatible procedures.
Specifications
InhibitorsPhosphatase Inhibitor
Quantity5 x 1 mL
Reagent TypePhosphatase Inhibitor
FormLiquid
Product LineHalt
Product TypePhosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store product at 4°C. Do not freeze.