PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)
PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)
Citations & References (8)
Invitrogen™

PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)

Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cellRead more
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Catalog NumberQuantity
A10070100 μg
Catalog number A10070
Price (EUR)
434,00
Each
Add to cart
Quantity:
100 μg
Price (EUR)
434,00
Each
Add to cart
Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Fluorogenic Phospholipase A Substrates Specifications:

• Label (Ex/Em): PED-A1
• Specificity: PLA1
• Cleavage product: D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry

Find More Probes for Lipid Metabolism and Fatty Acid Analogs
Review Fatty Acid Analogs and Phospholipids—Section 13.2 and Probes for Lipid Metabolism and Signaling—Section 17.4 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityCell-Permeant
For Use With (Equipment)Flow Cytometer, Microplate Reader
Product LineBODIPY
Quantity100 μg
Shipping ConditionRoom Temperature
SubstratePhospholipase A1 selective substrate
Detection MethodFluorescence
FormSolid
Substrate PropertiesLipid-Based Substrate
Target EnzymePhospholipase
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Citations & References (8)

Citations & References
Abstract
In vitro analysis of LIPH mutations causing hypotrichosis simplex: evidence confirming the role of lipase H and lysophosphatidic acid in hair growth.
Authors:Pasternack SM, von Kügelgen I, Müller M, Oji V, Traupe H, Sprecher E, Nöthen MM, Janecke AR, Betz RC,
Journal:J Invest Dermatol
PubMed ID:19536142
'Hypotrichosis simplex (HS) is a group of isolated alopecias that can be inherited as an autosomal-dominant or an autosomal-recessive trait. Hair loss usually begins in early childhood, and is diffuse and progressive. Mutations in LIPH, which encodes lipase member H, have recently been shown to cause an autosomal-recessive form of ... More
A novel fluorogenic substrate for the measurement of endothelial lipase activity.
Authors:Darrow AL, Olson MW, Xin H, Burke SL, Smith C, Schalk-Hihi C, Williams R, Bayoumy SS, Deckman IC, Todd MJ, Damiano BP, Connelly MA,
Journal:J Lipid Res
PubMed ID:21062953
'Endothelial lipase (EL) is a phospholipase A1 (PLA1) enzyme that hydrolyzes phospholipids at the sn-1 position to produce lysophospholipids and free fatty acids. Measurement of the PLA1 activity of EL is usually accomplished by the use of substrates that are also hydrolyzed by lipases in other subfamilies such as PLA2 ... More
Activation of a bacterial virulence protein by the GTPase RhoA.
Authors:Christen M, Coye LH, Hontz JS, LaRock DL, Pfuetzner RA, Megha, Miller SI,
Journal:Sci Signal
PubMed ID:19887681
The Rho family of guanosine triphosphatases (GTPases) are essential eukaryotic signaling molecules that regulate cellular physiology. Virulence factors from various pathogens alter the signaling of GTPases by acting as GTPase activating factors, guanine nucleotide exchange factors, or direct covalent modifiers; however, bacterial virulence factors that sense rather than alter the ... More
Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.
Authors:
Journal:Genet Med
PubMed ID:32336749
Structural, mechanistic, and physiological insights into phospholipase A-mediated membrane phospholipid degradation in Pseudomonas aeruginosa.
Authors:
Journal:Elife
PubMed ID:35536643
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