Primary Rat Cortex Neurons
Primary Rat Cortex Neurons
Gibco™

Primary Rat Cortex Neurons

Reliable rat cortical neurons in a convenient cryopreserved format provide an ideal source of neurons to enable neuroscience cell-based assays.
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Catalog NumberQuantity
A10840011 x 106 cells
A10840024 x 106
Catalog number A1084001
Price (EUR)
-
Quantity:
1 x 106 cells

Gibco Primary Rat Cortex Neurons (RCNs) are isolated and cryopreserved from day-18 Fischer 344 rat embryos. These cryopreserved RCNs offer a flexible, ready-to-use, and high-quality source of cells for neuronal experiments. They consistently yield post-thaw viability in the range of 50–80% and maintain high neuronal purity. Cryopreserved neurons are suitable for various applications requiring primary rat brain neurons, including patch-clamp electrophysiology, MEA, neurite outgrowth, synaptic protein expression, and neuron toxicity assays.

  • Convenience—these cryopreserved rat cortical neurons (RCNs) can be stored until needed and are ready to use when you plan your cell-based assays. They provide a convenient alternative to isolating neurons on your own.
  • Reliability—harvested from the cortex, these cryopreserved neurons meet rigorous quality standards to help ensure consistent performance
  • High Quality—every batch of Gibco Rat Cortex Neurons undergoes thorough testing, including viability assessment, immunofluorescence staining to confirm neuronal purity, sterility testing, and mycoplasma testing
  • Availability—our large-scale manufacturing capabilities allow us to support recurring orders of significant size and other requests, helping ensure you have the cells you need to complete your research studies

Culture Gibco Primary Rat Cortex Neurons with the B-27 Plus Neuronal Culture System to achieve the most robust performance.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Donor AttributesNormal
No. of Cells1 x 106
Product TypeRat Cortex Neuron
Quantity1 x 106 cells
Tested ForSterility, Viability, Mycoplasma
AgeFetal
Cell TypeNeural Cells
Culture TypeMammalian Cell Culture
FormCryopreserved
SpeciesRat
TissueCortex
Unit SizeEach
Contents & Storage
Store in liquid nitrogen, vapor-phase.

Frequently asked questions (FAQs)

What is the difference between Primary Rat Cortex Neurons, Sprague Dawley and Primary Rat Cortex Neurons?

These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.