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Applied Biosystems™
TaqMan™ Microbe Detection Assay
Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial speciesRead more
| Catalog Number | Quantity |
|---|---|
| A50137 | 250 μL |
Catalog number A50137
Price (EUR)
219,00
Each
Quantity:
250 μL
Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes. These assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.
Features of TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays include:
• Easy to use—just add DNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low-abundance microbial targets
• Accurate—identify microbial targets with high accuracy of quantitation
• Extensive content—over 300 pre-designed microbe detection assays and 100 pre-designed antibiotic resistance assays currently available
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
• Fast delivery—assays shipped in as little as 1–2 days (see below)
Approximate ship time
• Inventoried: 1–2 days in North America*
• Made to order: 4–6 days in North America
All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee.**
*Our most popular assays are stocked in North America for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.
**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.
Features of TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays include:
• Easy to use—just add DNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low-abundance microbial targets
• Accurate—identify microbial targets with high accuracy of quantitation
• Extensive content—over 300 pre-designed microbe detection assays and 100 pre-designed antibiotic resistance assays currently available
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
• Fast delivery—assays shipped in as little as 1–2 days (see below)
Approximate ship time
• Inventoried: 1–2 days in North America*
• Made to order: 4–6 days in North America
All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee.**
*Our most popular assays are stocked in North America for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.
**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.
Specifications
3'Primer ModificationFAM
5'Primer ModificationMGB-NFQ
FormatTube
No. of Reactions250 Reactions
Product LineTaqMan
Quantity250 μL
Shipping ConditionRoom temperature
Concentration20X
Detection MethodPrimer-probe
For Use With (Application)Pathogen Detection
Unit SizeEach
Contents & Storage
Store at -15°C to -25°C.