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Citations & References (16)
Invitrogen™
BODIPY™ FL Maleimide (BODIPY™ FL N-(2-Aminoethyl))Maleimide)
The thiol-reactive BODIPY™ FL maleimide produces electronically neutral dye conjugates that are spectrally similar to the negatively charged fluorescein dye.Read more
| Catalog Number | Quantity |
|---|---|
| B10250 | 5 mg |
Catalog number B10250
Price (EUR)
560,00
Each
Quantity:
5 mg
Price (EUR)
560,00
Each
The thiol-reactive BODIPY™ FL maleimide produces electronically neutral dye conjugates that are spectrally similar to the negatively charged fluorescein dye. This dye's lack of ionic charge results in minimal effects on the isoelectric points of standard proteins conjugated with this fluorophore. The small size and relatively long excited-state lifetime of BODIPY™ FL dye has proven useful for studying ligand-receptor interaction by fluorescence polarization. In addition, BODIPY™ FL dye has little or no spectral overlap with longer-wavelength dyes such as tetramethylrhodamine and Texas Red™ dye, making it a useful green fluorophore for multicolor applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityThiol
Label or DyeBODIPY™ FL
Product TypeMaleimide
Quantity5 mg
Reactive MoietyMaleimide
Shipping ConditionRoom Temperature
Label TypeBODIPY Dyes
Product LineBODIPY
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (16)
Citations & References
Abstract
Small vertical movement of a K+ channel voltage sensor measured with luminescence energy transfer.
Journal:Nature
PubMed ID:16094368
'Voltage-gated ion channels open and close in response to voltage changes across electrically excitable cell membranes. Voltage-gated potassium (Kv) channels are homotetramers with each subunit constructed from six transmembrane segments, S1-S6 (ref. 2). The voltage-sensing domain (segments S1-S4) contains charged arginine residues on S4 that move across the membrane electric
Quantitation of microparticles released from coated-platelets.
Journal:J Thromb Haemost
PubMed ID:16102115
'Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also
Evaluation of disulfide reduction during receptor-mediated endocytosis by using FRET imaging.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16950881
'Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of disulfide reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red to
An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly.
Journal:Nat Biotechnol
PubMed ID:16474383
'Virus assembly has not been routinely targeted in the development of antiviral drugs, in part because of the lack of tractable methods for screening in vitro. We have developed an in vitro assay of hepatitis B virus (HBV) capsid assembly, based on fluorescence quenching of dye-labeled capsid protein, for testing
The achondroplasia mutation does not alter the dimerization energetics of the fibroblast growth factor receptor 3 transmembrane domain.
Journal:Biochemistry
PubMed ID:16634636
The Gly380 --> Arg mutation in the TM domain of fibroblast growth factor receptor 3 (FGFR3) of the RTK family is linked to achondroplasia, the most common form of human dwarfism. The molecular mechanism of pathology induction is under debate, and two different mechanisms have been proposed to contribute to