D-Biotin
D-Biotin
Citations & References (19)
Invitrogen™

D-Biotin

D-biotin binds with high affinity to streptavidin, avidin and NeutrAvidin™ biotin-binding proteins.Read more
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Catalog NumberQuantity
B15951 g
B2065610 mL
Catalog number B1595
Price (EUR)
177,00
Each
Add to cart
Quantity:
1 g
Price (EUR)
177,00
Each
Add to cart
D-biotin binds with high affinity to streptavidin, avidin and NeutrAvidin™ biotin-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeBiotin (unconjugated)
Quantity1 g
Shipping ConditionRoom Temperature
ConjugateUnconjugated
FormSolid
Product LineInvitrogen
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

How should I resolubilize D-biotin (B20656)?

D-biotin may be resolubilized in DMF (1-2 mg/mL), DMSO (49 mg/mL), ethanol (<1 mg/mL) or a buffer with a pH between >6 and <9. For long-term stability, D-biotin is stable in aqueous buffers at moderately acidic to neutral pH.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (19)

Citations & References
Abstract
High-performance liquid chromatographic determination of biotin in biological materials after crown ether-catalyzed fluorescence derivatization with panacyl bromide.
Authors:Stein J, Hahn A, Lembcke B, Rehner G
Journal:Anal Biochem
PubMed ID:1534471
This paper reports a high-performance liquid chromatographic (HPLC) technique to determine biotin in biological samples. Biotin and the internal standard dethiobiotin are converted into fluorescent derivatives by using panacyl bromide [p-(9-anthroyloxy)phenacyl bromide] as a fluorescence label. Biotin is extracted from biological tissue with trichloroacetic acid and the extract is purified ... More
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Authors:Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells.
Authors:Rodriguez-Melendez R, Lewis B, McMahon RJ, Zempleni J
Journal:J Nutr
PubMed ID:12730407
'In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)gamma also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin ... More
Cellular uptake of biotin: mechanisms and regulation.
Authors:Said HM
Journal:J Nutr
PubMed ID:10064315
'This review describes our knowledge of biotin transport in the small intestine of humans and other mammals and presents recent findings in the area. Previous studies have shown that biotin transport across the brush border membrane of the small intestinal absorptive cells occurs via a carrier-mediated, Na+ gradient-dependent, electroneutral mechanism. ... More
Biotin transport through the blood-brain barrier.
Authors:Spector R, Mock D
Journal:J Neurochem
PubMed ID:3098919
'The unidirectional influx of biotin across cerebral capillaries, the anatomical locus of the blood-brain barrier, was measured with an in situ rat brain perfusion technique employing [3H]biotin. Biotin was transported across the blood-brain barrier by a saturable system with a one-half saturation concentration of approximately 100 microM. The permeability-surface area ... More
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