JC-9 Dye (Mitochondrial Membrane Potential Probe)

The ratiometric mitochondrial membrane potential probe JC-9 exhibits green fluorescence that is independent of membrane potential and an increase in red fluorescence at hyperpolarized membrane potentials.

Given that JC-1 and JC-9 dyes are mitochondrial membrane potential indicators, they are designed to be used in live cells with active mitochondria. These dyes are not compatible with fixed cell staining.