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Citations & References (9)
Invitrogen™
EnzChek™ Elastase Assay Kit
The EnzChek Elastase Assay Kit provides a sensitive, convenient and fast fluorometric method for measuring elastase or other protease activityRead more
| Catalog Number | Quantity |
|---|---|
| E12056 | 600 Assays |
Catalog number E12056
Price (EUR)
794,00
Each
Quantity:
600 Assays
Price (EUR)
794,00
Each
The EnzChek Elastase Assay Kit provides a sensitive, convenient and fast fluorometric method for measuring elastase or other protease activity in purified enzyme systems, cell/tissue lysates or for screening inhibitors in a high-throughput format. The substrate in the EnzChek kit is our BODIPY-FL-labeled DQ elastin conjugate that is highly labeled so that the fluorescence signal is quenched until enzymatic digestion yields highly fluorescent fragments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
Quantity600 Assays
Shipping ConditionRoom Temperature
Substrate PropertiesProtein-Based Substrate
Substrate TypeElastase Substrate
Target EnzymeElastases
For Use With (Application)Elastase Assay
Product LineEnzChek
Product TypeEnzChek Assay Kit
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (9)
Citations & References
Abstract
Genetic and biochemical characterization of PrtA, an RTX-like metalloprotease from Photorhabdus.
Journal:Microbiology
PubMed ID:12777498
'Proteases play a key role in the interaction between pathogens and their hosts. The bacterial entomopathogen Photorhabdus lives in symbiosis with nematodes that invade insects. Following entry into the insect, the bacteria are released from the nematode gut into the open blood system of the insect. Here they secrete factors
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Alveolar wall apoptosis causes lung destruction and emphysematous changes.
Journal:Am J Respir Cell Mol Biol
PubMed ID:12707011
'Pulmonary emphysema is characterized by alveolar wall destruction and airspace enlargement. Recent evidence indicates that epithelial or endothelial apoptosis may be involved in the pathogenesis of emphysema. Here, we describe the induction of emphysematous changes, including airspace enlargement, alveolar wall destruction, and enhanced lung distensibility, in mice receiving a single
A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens.
Journal:Microbiology
PubMed ID:12177336
The entomopathogen Photorhabdus luminescens secretes many proteins during the late stages of insect larvae infection and during in vitro laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of P. luminescens. PrtA is secreted via a classical type I secretory pathway
Changes in neutrophil functions in astronauts.
Journal:Brain Behav Immun
PubMed ID:15265537
Exploration class human spaceflight missions will require astronauts with robust immune systems. Innate immunity will be an essential element for the healthcare maintenance of astronauts during these lengthy expeditions. This study investigated neutrophil phagocytosis, oxidative burst, and degranulation of 25 astronauts after four space shuttle missions and in nine healthy