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Citations & References (30)
Invitrogen™
Epidermal Growth Factor, Biotin-XX Conjugate (biotin EGF)
This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains aRead more
| Catalog Number | Quantity |
|---|---|
| E3477 | 20 μg |
Catalog number E3477
Price (EUR)
846,00
Each
Quantity:
20 μg
Price (EUR)
846,00
Each
This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains a long spacer arm that enhances the probe’s affinity for the EGF receptor and facilitates binding of a label such as a dye–streptavidin molecule, a Qdot™ nanocrystals–streptavidin molecule, or an enzyme-conjugated streptavidin. Labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.
Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1
Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1
Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Label or DyeBiotin & Other Haptens
Quantity20 μg
Shipping ConditionWet Ice
FormSolid
Product TypeGrowth Factor
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Citations & References (30)
Citations & References
Abstract
Localization of EGF receptors in frozen tissue sections by antibody and biotinylated EGF-based techniques.
Journal:J Histochem Cytochem
PubMed ID:8308248
'We developed a sensitive EGF receptor detection method for frozen tissue sections using biotinylated EGF as the primary reagent. The method was directly compared with an immunohistochemical technique based on an anti-EGF receptor monoclonal antibody (MAb EGFR1) in normal human and rat tissues and in human tumors. The method was
PI3P signaling regulates receptor sorting but not transport in the endosomal pathway.
Journal:J Cell Biol
PubMed ID:12975344
'While evidence is accumulating that phosphoinositide signaling plays a crucial role in growth factor and hormone receptor down-regulation, this signaling pathway has also been proposed to regulate endosomal membrane transport and multivesicular endosome biogenesis. Here, we have followed the fate of the down-regulated EGF receptor (EGFR) and bulk transport (fluid
Retention of biologic activity of human epidermal growth factor following conjugation to a blood-brain barrier drug delivery vector via an extended poly(ethylene glycol) linker.
Journal:Bioconjug Chem
PubMed ID:9893961
'Human brain gliomas overexpress the receptor for epidermal growth factor (EGF), and radiolabeled EGF is a potential peptide radiopharmaceutical for imaging human brain tumors, should this peptide be made transportable through the blood-brain barrier (BBB) in vivo. Peptide drug delivery to the brain may be facilitated by conjugating peptide radiopharmaceuticals
In Alzheimer's disease the Golgi apparatus of a population of neurons without neurofibrillary tangles is fragmented and atrophic.
Journal:Am J Pathol
PubMed ID:8579105
'Recent immunocytochemical and morphometric studies in amyotrophic lateral sclerosis, Alzheimer's disease (AD), and aging indicate that the neuronal Golgi apparatus is a reliable index of activity or degeneration. To further evaluate a possible role of the Golgi apparatus in the pathogenesis of AD, we examined by double labeling the neuronal
Targeted cellular delivery of quantum dots loaded on and in biotinylated liposomes.
Journal:Bioconjug Chem
PubMed ID:20715851
'We describe the preparation, biophysical characterization, and receptor-mediated cellular internalization of biotinylated lipid particles (BLPs) loaded on the surface and internally with two distinct (colors) of quantum dot (QD) probes. BLPs were formulated with 1.4 and 2.7 mol % PEG-lipids containing either a fusogenic or pH-sensitive lipid to promote bilayer