EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
Citations & References (9)
Invitrogen™

EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid,Read more
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Catalog NumberQuantity
E66391 Kit
Catalog number E6639
Price (EUR)
718,00
Each
Add to cart
Quantity:
1 Kit
Price (EUR)
718,00
Each
Add to cart
The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.

See our complete line of Fluorescence Microplate assays.

  • Generalized mechanism allows for measurement of a variety of proteases
  • Can be used to continuously measure protease kinetics
  • Excitation/emission similar to Texas Red™ dye (589/617 nm)

    This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY™ TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY™ TR-X dye has excitation and emission spectra similar to those of the Texas Red™ fluorophore.

    • For Research Use Only. Not for use in diagnostic procedures.
    Specifications
    Detection MethodFluorescence Intensity
    Quantity1 Kit
    Shipping ConditionRoom Temperature
    Substrate PropertiesProtein-Based Substrate
    Substrate TypeProtease Substrate
    Target EnzymeProteases
    For Use With (Application)Protease Assay
    For Use With (Equipment)Fluorescence Microplate Reader
    Product LineEnzChek
    Product TypeEnzChek Assay Kit
    Unit SizeEach
    Contents & Storage
    Store in freezer -5°C to -30°C and protect from light.

    Frequently asked questions (FAQs)

    What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

    The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

    What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

    As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    How long is the reconstituted BODIPY substrate in the EnzChek Protease Assay Kit, red fluorescence, 100-1000 assays (Cat. No. E6639) stable?

    Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Citations & References (9)

    Citations & References
    Abstract
    Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.
    Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP
    Journal:Anal Biochem
    PubMed ID:9299009
    'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More
    Subnanoliter enzymatic assays on microarrays.
    Authors:Angenendt P, Lehrach H, Kreutzberger J, Glökler J
    Journal:Proteomics
    PubMed ID:15700241
    'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays ... More
    Continuous assay of proteases using a microtiter plate fluorescence reader.
    Authors:Menges DA, Ternullo DL, Tan-Wilson AL, Gal S
    Journal:Anal Biochem
    PubMed ID:9398357
    A protease-dependent mechanism for initiating T-dependent B cell responses to large particulate antigens.
    Authors:Catron DM, Pape KA, Fife BT, van Rooijen N, Jenkins MK,
    Journal:J Immunol
    PubMed ID:20208013
    Ab production is critical for antimicrobial immunity, and the initial step in this process is the binding of Ag to the BCR. It has been shown that small soluble proteins can directly access the lymph node follicles to reach naive B cells, but virus particles must be translocated into follicles ... More
    Selective modulation of superantigen-induced responses by streptococcal cysteine protease.
    Authors:Kansal RG, Nizet V, Jeng A, Chuang WJ, Kotb M
    Journal:J Infect Dis
    PubMed ID:12552423
    Streptococcal pyrogenic exotoxin (Spe) B, a streptococcal cysteine protease, is believed to be important in group A streptococcal (GAS) pathogenesis. The present study examined the effect of SpeB on the activity of superantigenic exotoxins secreted by M1T1 GAS isolates. The proliferative response of human lymphocytes to culture supernatant (SUP) from ... More
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