NAD⁺/NADH Colorimetric Assay Kit

Detection of total content of NAD+ and NADH

With this kit, ethanol generates acetaldehyde under the action of an enzyme. Meanwhile, NAD+ is reduced to NADH. NADH, under the action of hydrogen transmitter, transfers electrons to WST-8 to produce a yellow product that has a characteristic absorption peak at 450 nm. The total content of NAD+ and NADH can be quantified by measuring the OD value at 450 nm.

Detect NADH

After treating the sample, it is heated at 60°C. The NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form a yellow product, and the amount of NADH is determined by measuring the OD value at 450 nm.

Detect NAD+ and NAD+/NADH

The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained from the first two steps as well as the separate content of NADPH.

Note: NADP+ and NADPH have no effect on the determination results.

This kit is useful for researchers studying cellular metabolism and redox biology.

Key features

• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results