Heparin, Fluorescein Conjugate
Heparin, Fluorescein Conjugate
Citations & References (42)
Invitrogen™

Heparin, Fluorescein Conjugate

The green-fluorescent fluorescein heparin conjugate should be a useful tool for studying binding of this mucopolysaccharide in cells and tissue.Read more
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Catalog NumberQuantity
H74821 mg
Catalog number H7482
Price (EUR)
488,00
Each
Add to cart
Quantity:
1 mg
Price (EUR)
488,00
Each
Add to cart
The green-fluorescent fluorescein heparin conjugate should be a useful tool for studying binding of this mucopolysaccharide in cells and tissue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label TypeClassic Dyes
Protein SubtypeOther Proteins
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateFluorescein
FormLyophilized
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

How can I dissolve Heparin, Fluorescein Conjugate (Cat. No. H7482)?

The molecular weight of Heparin, Fluorescein Conjugate (Cat. No. H7482) is ~18,000 and the information can be found on the Calcium Regulation—Section 17.2 page (see Data Table).

Heparin is quite soluble in water (10mg/ml) or aqueous buffer and you can prepare stock solutions of at least 1 mM using water as the solvent. Once dissolved, the stability of the nonsterile solution is very poor, so it should either be sterile filtered (0.2 µm) or an antibacterial (e.g., 0.06-1% benzyl alcohol) should be added. Heparin in solution should not be frozen, but is stable at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .

What is size of the Heparin, Fluorescein Conjugate (Cat. No. H7482)?

Heparin is an irregular aggregate of a 3,000 MW monomers, and they are low molecular weight heparins. They are purified by dialysis with a cut off of > 10 kDa and have an average of 18kDa.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .

Citations & References (42)

Citations & References
Abstract
Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death.
Authors:Berry D, Lynn DM, Sasisekharan R, Langer R
Journal:Chem Biol
PubMed ID:15123243
'Heparin/heparan sulfate-like glycosaminoglycans (HSGAGs) are involved in diverse cellular processes in the extracellular matrix (ECM). The biological effect of HSGAGs depends on disaccharide content and physiological location within the ECM. HSGAGs are also brought into cells during membrane transcytosis and growth factor signaling while protein bound. We sought to probe ... More
Solid-phase synthesis of fluorescent heparin.
Authors:Radoff S, Danishefsky I
Journal:Anal Biochem
PubMed ID:7091663
Heparin binding is necessary, but not sufficient, for fibronectin aggregation. A fluorescence polarization study.
Authors:Bentley KL, Klebe RJ, Hurst RE, Horowitz PM
Journal:J Biol Chem
PubMed ID:3997865
'Analysis of parameters governing heparin binding to fibronectin indicates that heparin binding is a necessary, but insufficient, condition for fibronectin cryoprecipitation. Heparin binding to fibronectin is a rapid, readily reversible event which can occur under several conditions which prohibit fibronectin cryoprecipitation. While cryoprecipitation of fibronectin is abolished at temperatures in ... More
Binding and internalization of heparin by vascular smooth muscle cells.
Authors:Castellot JJ, Wong K, Herman B, Hoover RL, Albertini DF, Wright TC, Caleb BL, Karnovsky MJ
Journal:J Cell Physiol
PubMed ID:3930515
'Previous work from our laboratory has demonstrated that heparin specifically inhibits the proliferation of vascular smooth muscle cells in vivo and in vitro. In this paper, we examine the binding and mode of internalization of heparin by smooth muscle cells. For these studies, radiolabeled and fluoresceinated (FITC) heparin probes were ... More
Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.
Authors:Busby TF, Argraves WS, Brew SA, Pechik I, Gilliland GL, Ingham KC
Journal:J Biol Chem
PubMed ID:7629186
'The thirteenth type III domain of fibronectin binds heparin almost as well as fibronectin itself and contains a so-called heparin-binding consensus sequence, Arg6-Arg7-Ala8-Arg9 (residues 1697-1700 in plasma fibronectin). Barkalow and Schwarzbauer (Barkalow, F.J., and Schwarzbauer, J.E. (1991) J. Biol. Chem. 266, 7812-7818) showed that mutation of Arg6-Arg7 in domain III-13 ... More
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