LIVE/DEAD™ Sperm Viability Kit
LIVE/DEAD™ Sperm Viability Kit
Citations & References (69)
Invitrogen™

LIVE/DEAD™ Sperm Viability Kit

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm byRead more
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Catalog NumberQuantity
L70111 kit
Catalog number L7011
Price (EUR)
904,00
Each
Add to cart
Quantity:
1 kit
Price (EUR)
904,00
Each
Add to cart
The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR® 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant propidium iodide labels the nucleic acids of membrane-compromised sperm with red fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEukaryotic Cells, Sperm Cells
DescriptionLIVE/DEAD™ Sperm Viability Kit
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Quantity1 kit
Shipping ConditionRoom Temperature
ColorGreen, Red
Emission516/617
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineLIVE/DEAD
Product TypeSperm Viability Kit
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (69)

Citations & References
Abstract
Seminal plasma addition attenuates the dilution effect in bovine sperm.
Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH
Journal:Theriogenology
PubMed ID:11467516
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV
Journal:Biol Reprod
PubMed ID:11870056
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
Authors:Chalah T, Brillard JP
Journal:Theriogenology
PubMed ID:10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M
Journal:Anim Reprod Sci
PubMed ID:12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
Journal:Cytometry
PubMed ID:10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
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