MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)

Citations & References (280)

Invitrogen™

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)

MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTTRead more

Catalog Number	Quantity
M6494	1 g

Catalog number M6494

Price (EUR)

214,65

Online Exclusive

228,00

Save 13,35 (6%)

Add to cart

Quantity:

Price (EUR)

214,65

Online Exclusive

228,00

Save 13,35 (6%)

Add to cart

MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

DescriptionMTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)

Detection MethodAbsorbance

Dye TypeColorimetric Reagent-Based

Quantity1 g

Shipping ConditionRoom Temperature

Product LinePierce

Product TypeMTT

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to use MTT to determine cell proliferation. Do I really need a standard curve? Will the assay tell me percent of live versus dead cells?

MTT is reduced only by live cells and the rate of dye reduction may vary from cell to cell based on their life cycle stage, age, health and other factors. This assay would not reveal the number of dead cells or a ratio of live to dead cells. A standard curve is recommended to determine an approximate number of live cells per sample.

To obtain a ratio of live to dead cells, use a two color fluorescent assay, such as LIVE/DEAD Viability/Cytotoxicity Kit (Cat. No. L3224) or differences in intensity of a single fluorescent color, using a flow cytometer and the LIVE/DEAD Fixable Kits.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (280)

Citations & References

Abstract

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Authors:

Journal:

PubMed ID:10896672

Detection of low copy numbers of HPV DNA by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction.

Authors:Lizard G, Chignol MC, Souchier C, Roignot P, Chardonnet Y, Schmitt D

Journal:J Virol Methods

PubMed ID:9672129

'In genital lesions infected by human papillomavirus (HPV), histological criteria and HPV DNA typing are of prognostic value. Therefore, non-radioactive methods such as in situ hybridization are used extensively since they preserve the histological organization of the tissue, and allow the detection and characterization of HPV DNA. However, the sensitivity ... More

Identification of an amino acid residue in multidrug resistance protein 1 critical for conferring resistance to anthracyclines.

Authors:Zhang DW, Cole SP, Deeley RG

Journal:J Biol Chem

PubMed ID:11278596

'Murine multidrug resistance protein 1 (mrp1), unlike human MRP1, does not confer resistance to anthracyclines. Previously, we have shown that a human/murine hybrid protein containing amino acids 959-1187 of MRP1 can confer resistance to these drugs. We have now examined the functional characteristics of mutant proteins in which we have ... More

Oxidative stress induces intracellular accumulation of amyloid beta-protein (Abeta) in human neuroblastoma cells.

Authors:Misonou H, Morishima-Kawashima M, Ihara Y

Journal:Biochemistry

PubMed ID:10841777

'Several lines of evidence suggest that enhanced oxidative stress is involved in the pathogenesis and/or progression of Alzheimer''s disease (AD). Amyloid beta-protein (Abeta) that composes senile plaques, a major neuropathological hallmark of AD, is considered to have a causal role in AD. Thus, we have studied the effect of oxidative ... More

View all MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) citations