Qdot™ 525 ITK™ Amino (PEG) Quantum Dots
Qdot™ 525 ITK™ Amino (PEG) Quantum Dots
Citations & References (4)
Invitrogen™

Qdot™ 525 ITK™ Amino (PEG) Quantum Dots

Qdot™ 525 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostableRead more
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Catalog NumberQuantity
Q21541MP250 μL
Catalog number Q21541MP
Price (EUR)
892,65
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1.190,00
Save 297,35 (25%)
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Quantity:
250 μL
Price (EUR)
892,65
Online Exclusive
1.190,00
Save 297,35 (25%)
Each
Add to cart
Qdot™ 525 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostable fluorescently labeled proteins or other biopolymers. These probes are functionalized with amine-derivatized PEG, which prevents non-specific interactions and provides a convenient handle for conjugation. The amino quantum dots react efficiently with isothiocyanates and succinimidyl esters, or with native carboxylic acids using water-soluble carbodiimides such as EDC. Such derivatives may be used for various labeling and tracking applications that require ultrabright and stable fluorescence. Our Qdot™ ITK™ amino quantum dots are provided as 8 μM solutions and are available in 8 colors of Qdot™ probes.

Important Features of Qdot™ ITK™ Amino Quantum Dots:
• Qdot™ 525 ITK™ amino quantum dot has emission maxima of ∼525 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications

Properties of Qdot™ Nanocrystals
Qdot™ probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot™ probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot™ labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.

About the Innovator’s Tool Kit Qdot™ ITK™ Reagents
These Qdot™ ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.

Other Forms of Qdot™ Nanocrystals are Available
In addition to the amine-derivatized form, we offer Qdot™ ITK™ quantum dots with carboxyl and aliphatic hydrocarbon modifications. We’ve also developed a wide range of Qdot™ nanocrystals conjugates and labeling kits. Investigate the properties of Qdot™ nanocrystals or read the Molecular Probes™ Handbook Section 6.6—Qdot™ Nanocrystals to find out more.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde
Concentration8 μM
Emission525
Label or DyeQdot™ 525
Product TypeQuantum Dot
Quantity250 μL
Reactive MoietyAmine, Primary Amine
Shipping ConditionRoom Temperature
Label TypeQdot Nanocrystals
Product LineITK, Qdot
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

How large are the Qdot nanocrystals?

The core/shells are only a few nanometers in diameter (some are elliptical), but with the outer polymer coatings, a fully-functionalized Qdot nanocrystal can range from 15 to 21 nm in hydrodynamic diameter, similar in size to some proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 525 ITK™ Amino (PEG) Quantum Dots FAQs

Citations & References (4)

Citations & References
Abstract
Sensitive quantification of PEGylated compounds by second-generation anti-poly(ethylene glycol) monoclonal antibodies.
Authors:Su YC, Chen BM, Chuang KH, Cheng TL, Roffler SR,
Journal:Bioconjug Chem
PubMed ID:20536171
'Poly(ethylene glycol) (PEG) is often attached to compounds to increase serum half-life, reduce immunogenicity, and enhance bioavailability. Accurate and sensitive quantification of PEG conjugates is critical for product development, pharmacokinetic measurements, and efficacy studies. However, PEGylated compounds can be difficult to quantify due to epitope masking by PEG. We previously ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Authors:Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
Journal:ACS Nano
PubMed ID:23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
microPET-based biodistribution of quantum dots in living mice.
Authors:Schipper ML, Cheng Z, Lee SW, Bentolila LA, Iyer G, Rao J, Chen X, Wu AM, Weiss S, Gambhir SS,
Journal:J Nucl Med
PubMed ID:17704240
This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. (64)Cu-Labeled 800- or 525-nm emission wavelength QD (21- or 12-nm diameter), with or without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting ... More
Qdot nanobarcodes for multiplexed gene expression analysis.
Authors:Eastman PS, Ruan W, Doctolero M, Nuttall R, de Feo G, Park JS, Chu JS, Cooke P, Gray JW, Li S, Chen FF
Journal:Nano Lett
PubMed ID:16683851
We report a quantum dot (Qdot) nanobarcode-based microbead random array platform for accurate and reproducible gene expression profiling in a high-throughput and multiplexed format. Four different sizes of Qdots, with emissions at 525, 545, 565, and 585 nm are mixed with a polymer and coated onto the 8-mum-diameter magnetic microbeads ... More