SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Citations & References (6)
Invitrogen™

SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because theRead more
Have Questions?
Catalog NumberQuantity
S11351250 μL
Catalog number S11351
Price (EUR)
510,00
Each
Add to cart
Quantity:
250 μL
Price (EUR)
510,00
Each
Add to cart

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11350) to enable researchers to find the dye that works best for a particular application.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed, Blue
DescriptionSYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Detection MethodFluorescence
Dye TypeCell-Permeant
Emission441 nm
Excitation Wavelength Range420 nm
For Use With (Equipment)Fluorescence Microscope
Product LineSYTO
Quantity250 μL
Shipping ConditionRoom Temperature
Volume (Metric)250 μL
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (6)

Citations & References
Abstract
Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.
Authors:Pin S, Chen H, Lein PJ, Wang MM
Journal:J Neurochem
PubMed ID:16441515
'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid.
Authors:Amari DT, Marques CN, Davies DG,
Journal:
PubMed ID:23935049
'In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer cis-2-decenoic acid in the human pathogen Pseudomonas aeruginosa. The protein is encoded by PA14_54640 (PA0745), named dspI for dispersion inducer. The gene sequence for this ... More
Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.
Authors:Khan MM, Pyle BH, Camper AK,
Journal:Appl Environ Microbiol
PubMed ID:20543046
An issue of critical concern in microbiology is the ability to detect viable but nonculturable (VBNC) and viable-culturable (VC) cells by methods other than existing approaches. Culture methods are selective and underestimate the real population, and other options (direct viable count and the double-staining method using epifluorescence microscopy and inhibitory ... More
Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell.
Authors:Pucovský V, Harhun MI, Povstyan OV, Gordienko DV, Moss RF, Bolton TB,
Journal:J Cell Mol Med
PubMed ID:17760838
This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of guinea-pig mesenteric arteries and single AIL cells freshly isolated ... More
Subcutaneous Injections of Nanofat Adipose-derived Stem Cell Grafting in Facial Rejuvenation.
Authors:
Journal:Plast Reconstr Surg Glob Open
PubMed ID:32095390
View all SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO citations