SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Citations & References (5)

Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

The Alexa Fluor™ 680 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree ofRead more

Catalog Number	Quantity
S30045	1 kit

Catalog number S30045

Price (EUR)

978,00

Add to cart

Quantity:

1 kit

Price (EUR)

978,00

Add to cart

The Alexa Fluor™ 680 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree of labeling for in vivo imaging applications (DOL; ∼2) over a 6-fold antibody concentration range with no adjustments in reaction volume, dye concentration, or antibody concentration necessary. Using this procedure, optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injection into animals.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorInfrared

Detection MethodFluorescence

Excitation/Emission679/702

Label TypeAlexa Fluor Dyes

Labeling Scale0.5 to 3 mg

Product LineAlexa Fluor, SAIVI

Product TypeLabeling Kit

Quantity1 kit

Shipping ConditionRoom Temperature

Labeling TargetAntibodies

Label or DyeAlexa Fluor 680

Unit SizeEach

Contents & Storage

Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.

Authors:Gaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,

Journal:

PubMed ID:24469819

'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More

Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.

Authors:Karlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22101523

'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More

Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.

Authors:McCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,

Journal:Blood

PubMed ID:23243270

Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More

Influenza virus aerosol exposure and analytical system for ferrets.

Authors:Gustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21536880

Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More