Gateway™ pET-DEST42 Vector
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Citas y referencias (3)
Invitrogen™

Gateway™ pET-DEST42 Vector

El vector de destino Champion™ pET-DEST42 Gateway™ está diseñado para la clonación rápida con un clon de entrada Gateway™ queMás información
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Número de catálogoCantidad
122760106 μg
Número de catálogo 12276010
Precio (EUR)
1.494,00
Each
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Cantidad:
6 μg
Precio (EUR)
1.494,00
Each
Añadir al carro de la compra
El vector de destino Champion™ pET-DEST42 Gateway™ está diseñado para la clonación rápida con un clon de entrada Gateway™ que utiliza la recombinación específica de sitio de fago lambda y la expresión procariota de alto nivel posterior controlada por el potente promotor T7 bacteriófago. La expresión es inducida por la producción de ARN polimerasa T7 en BL21(DE3) E. coli. El vector de destino Champion™ pET-DEST42 ofrece:

• Promotor T7lac bacteriófago para expresión de alto nivel
Secuencias de operador O para la unión del represor de lac a fin de garantizar un mayor estrechamiento regulación de la transcripción
• Origen pBR322 para una expresión basal mínima
• Etiquetas C-terminal V5 y 6xHis para una detección y purificación eficaces
Sitios R para una recombinación eficaz con cualquier vector de entrada Gateway™ flanqueado por attL
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Resistencia bacteriana a los antibióticosAmpicilina (AMPR)
Sistema constitutivo o inducibleInducible
Agente inductorIPTG
Tipo de productoVector de expresión de destino del sistema Gateway
Cantidad6 μg
Agente de selección (eucariótico)Ninguno
VectorpET, pDEST
Método de clonaciónGateway
Línea de productosGateway
PromotorT7, lacO
Etiqueta de proteínaEtiqueta His (6x), Etiqueta de epítopo V5
Unit SizeEach
Contenido y almacenamiento
El vector de destino Champion™ pET-DEST42 Gateway™ incluye 6μg de vector. Conservar a -20°C. Se garantiza la estabilidad de este vector durante 6 meses si se almacena adecuadamente.

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pET-DEST42 Vector FAQs

Citations & References (3)

Citations & References
Abstract
Engineering vitamin E content: from Arabidopsis mutant to soy oil.
Authors:Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL,
Journal:Plant Cell
PubMed ID:14630966
'We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of delta-tocopherol and decreased gamma-tocopherol in the seed. Enzyme assays ... More
bZIP transcription factor interactions regulate DIF responses in Dictyostelium.
Authors:Huang E, Blagg SL, Keller T, Katoh M, Shaulsky G, Thompson CR,
Journal:Development
PubMed ID:16410410
The signalling molecule DIF-1 is required for normal cell fate choice and patterning in Dictyostelium. To understand how these developmental processes are regulated will require knowledge of how cells receive and respond to the DIF-1 signal. Previously, we have described a bZIP transcription factor, DimA, which is required for cells ... More
Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family.
Authors:Canaan S, Maurin D, Chahinian H, Pouilly B, Durousseau C, Frassinetti F, Scappuccini-Calvo L, Cambillau C, Bourne Y,
Journal:Eur J Biochem
PubMed ID:15373841
The Mycobacterium tuberculosis genome contains an unusually high number of proteins involved in the metabolism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c ... More