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Invitrogen™

eBioscience™ Streptavidin eFluor™ 615 Conjugate

Utilizados habitualmente en protocolos de tinción indirecta para detectar anticuerpos primarios biotinilados en aplicaciones de inmunohistoquímica e inmunocitoquímica. La estreptavidina tiene gran afinidad a la unión con la biotina.
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Número de catálogoCantidad
42-4317-82100 μg
42-4317-8025 μg
Número de catálogo 42-4317-82
Precio (EUR)
490,00
Each
Cantidad:
100 μg
Precio (EUR)
490,00
Each
Los conjugados de estreptavidina y fluorocromo se utilizan habitualmente en protocolos de tinción indirecta para detectar anticuerpos primarios biotinilados en aplicaciones de inmunohistoquímica e immunocitoquímica. La estreptavidina tiene gran afinidad a la unión con la biotina.

Emisión
615 nm

Excitación
595 nm

Aplicación declarada
Tinción inmunocitoquímica e inmunohistoquímica de secciones de tejido congeladas, tinción inmunohistoquímica de secciones de tejido incrustadas de parafina fija en formol

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración0,2 mg/ml
Tipo de productoConjugado de estreptavidina (fluorescente)
Cantidad100 μg
Molécula dianaBiotina
ConjugadoeFluor 615
FormularioLíquido
Línea de productoseBioscience
Unit SizeEach

Preguntas frecuentes

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.