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Applied Biosystems™

7500 Real-Time PCR System for Human Identification with Dell Desktop GX620

The Applied Biosystems 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternityMás información
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Número de catálogoCantidad
43666041 System
Número de catálogo 4366604
Precio (EUR)
-
Cantidad:
1 System
The Applied Biosystems 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Desktop GX620 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile program that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM⁄SYBR Green I, VIC⁄JOE, TAMRA⁄CY3, ROX⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.

Complete Validated Solution
Applied Biosystems offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.

EXPERIENCE MATTERS™
When you partner with Applied Biosystems you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

For Research Use Only. Not for use in diagnostics procedures.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Tinte calibradoSYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
DimensionesWidth 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)
Intervalo dinámico9 logs
Requisitos eléctricos950 W
Ordenador externo16XDVD+/-RW, 80 GB HD, 1 GB RAM, Pentium IV 630/3 GHz, Dell OptiPlex GX620 Minitower
Para utilizar con (equipo)Sistema 7500
Formato96-well Plate
Compatibilidad de alto rendimientoMultiplexado
Sistema ópticoCCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Colorante de referencia pasivaROX (tubo independiente), No ROX, ROX (premezclado)
Velocidad máxima de rampa de bloque2.5°C⁄s
Precisión99.7% Confidence (5000 & 10000 Copies)
Línea de productosPrimer Express
Cantidad1 System
Velocidad de reacciónEstándar
Intervalo de volumen de reacción20-100 μl
Velocidad de rampa de la muestra± 1.1°C⁄s
Sensibilidad1 Copy of RNase P gene (Human Genomic DNA)
Compatibilidad del softwareGeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1
Intervalo de temperatura (métrico)De 4 a 100 °C
Exactitud térmica0.25°C (35°C to 95°C after 3 min.)
Sistema de termocicladoSistema basado en Peltier
Uniformidad térmica0.50°C (after 30 s)
Capacidad de procesamiento4 to 5-96-well Plates per 8-hr day
Tensión120 V
Peso75 lb.
Unit SizeEach
Contenido y almacenamiento
Ambient

Preguntas frecuentes

What run conditions should I use for the TaqMan® Advanced miRNA assays with the discontinued 7900HT Real-Time PCR System?

We recommend using the same thermal profile as listed for the 7500 and 7500 Fast systems (Table 8) in the TaqMan® Advanced miRNA Assays User Guide: https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2F100027897_TaqManAdv_miRNA_Assays_UG.pdf