Applied Biosystems™

™ Kit de preparación rápida de muestras de prepSEQ con proteinasa K

Número de catálogo: 4426714
Applied Biosystems™

™ Kit de preparación rápida de muestras de prepSEQ con proteinasa K

Número de catálogo: 4426714
Número de catálogo
4426714
Tamaño de la unidad
1 kit
Precio (EUR)
Disponibilidad
-
Cantidad
Número de catálogoTamaño de la unidadPrecio (EUR)DisponibilidadCantidad
44267141 kit
-
Descripción general del producto
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The PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

Especificaciones

Formato
Columna de centrifugación
Isolation Technology
Columna de centrifugación, filtración gruesa, enzimática
Tipo de muestra
Muestras líquidas (p. ej., suero), muestras ambientales y alimentarias, cultivo bacteriano, bacterias (difíciles de lisar)
Tipo de producto final
ADN bacteriano
Para utilizar con (equipo)
Centrífuga
Para utilizar con (aplicación)
PCR en tiempo real cuantitativa (PCRc), PCR
Compatibilidad de alto rendimiento
No compatible con alto rendimiento (manual)
N.º de reacciones
100 reacciones
Línea de productos
PrepSEQ™
Objetivo
ADN genómico
Cantidad de material de partida
750 µl

Contenido y almacenamiento

Cada kit contiene 100 columnas de centrifugación, 100 tubos de microcentrífuga, tampón de lisis (almacene a 4 °C) y proteinasa K (almacene a -20 °C). Se pueden preparar 100 muestras con este kit.)

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