Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml
Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml
Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml
Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml
Citas y referencias (4)
Thermo Scientific™

Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml

Los concentradores de proteínas de PES Thermo Scientific™ Pierce™ son dispositivos de ultrafiltración centrífuga desechables con una membrana de polietersulfonaMás información
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Número de catálogoCantidadVolumen de muestra
8853124 concentradoresDe 5 a 20 ml
8850225 concentradores≤ 0,5ml
8852110 concentradoresDe 2 a 6 ml
8852224 concentradoresDe 2 a 6 ml
8852910 concentradoresDe 5 a 20 ml
885364 concentradoresDe 20 a 100 ml
Número de catálogo 88531
Precio (EUR)
347,00
Each
Añadir al carro de la compra
Cantidad:
24 concentradores
Volumen de muestra:
De 5 a 20 ml
Precio (EUR)
347,00
Each
Añadir al carro de la compra
Los concentradores de proteínas de PES Thermo Scientific™ Pierce™ son dispositivos de ultrafiltración centrífuga desechables con una membrana de polietersulfona (PES) para la concentración, la desalinización y el intercambio de tampones de muestras biológicas.Este producto tiene un tope de peso molecular (MWCO) de 30K y se puede utilizar para el procesamiento de volúmenes de muestra de 5 a 20 ml.

Características de los concentradores de proteínas de PES Pierce:

•Concentración proteica rápida: puede conseguirse una concentración de la muestra de 10 a 30 veces en un periodo de 5 a 30 minutos para MWCO de 10K (el tiempo puede variar para un producto con un MWCO diferente)
• Excelente recuperación de proteínas: la recuperación de proteínas típica es >90 %
• Versatilidad: concentración de muestras sencillo, diafiltración o intercambio de tampones de muestras biológicas
• Compatibilidad: puede usarse tanto en rotores de ángulo fijo como en rotores de vasos basculantes; recoger y recuperar muestras sin centrifugación inversa

Para facilitar la identificación, el valor de MWCO se graba en el lateral de cada dispositivo.Para facilitar la adición y recuperación de muestras, hay una ventana transparente con graduaciones en el lateral de cada dispositivo, lo que permite la determinación visual del volumen de retención.El diseño vertical de la membrana permite altas velocidades de flujo, baja unión no específica y una agregación proteica insignificante en la membrana; y proporciona resultados fiables y coherentes.

Los concentradores de proteínas de PES Pierce contienen una membrana clasificada para retener moléculas con un peso molecular al menos 2 veces mayor que la clasificación MWCO de la membrana de PES del dispositivo.Una menor recuperación puede ocurrir cuando se usa un concentrador con moléculas más pequeñas que la masa recomendada.La recuperación de proteínas variará dependiendo de la proteína específica de la muestra y de su concentración inicial.Para lograr una recuperación >90 % de la proteína, la concentración mínima de la muestra de proteína debe ser de 0,1 mg/ml.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Línea de productosPierce
Diana de purificaciónproteína
Cantidad24 concentradores
Condiciones de envíoTemperatura ambiente
TipoFiltro
DesechablesSí
FormatoColumna de centrifugación cónica de 50 ml
MWCO30 kDa
Material (membrana)PES
Volumen de muestraDe 5 a 20 ml
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente.

Preguntas frecuentes

Can I autoclave PES protein concentrators?

We do not recommend autoclaving the PES protein concentrators. High temperatures will significantly increase the membrane molecular weight cutoff (MWCO). To sterilize, we recommend using a 70% ethanol solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use PES protein concentrators for desalting and buffer exchange?

Yes, the PES membrane is compatible with desalting and buffer exchange. Protocols for concentrating, desalting, and buffer exchange only are provided in the product manuals.

How do PES protein concentrators work?

The protein concentrator columns have an upper compartment that holds the sample, and a lower compartment. The two compartments are separated by a polyethersulfone (PES) semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The protein concentrator cassettes also contain a PES membrane and a unique channel design that ensures high optimal flux with no optimization effort. Unlike other systems, the fluid is passed parallel to the filter, rather than being pushed through a membrane perpendicularly, which can clog the filter. For maximum protein recovery in both columns and cassettes, samples should have a molecular weight two-fold greater than the molecular weight cutoff (MWCO) of the concentrator membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the different sizes of PES protein concentrators you offer?

Pierce polyethersulfone (PES) protein concentrators are offered in six distinct MWCOs of 3K, 5k, 10K, 30K, 50K, and 100K, and the cassettes are offered in five membrane MWCOs of 3K, 10K, 30K, 50K, and 100K. The columns can process four different sample volumes ranges of 100-500 µL, 2-6 mL, 5-20 mL, and 20-100 mL, while the cassettes can process 0.5-6 L. Please see our selection chart and video found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/protein-concentrators.html).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I labeled my protein with your kit, but now it‘s too dilute for my application. What should I do?

There are different ways to concentrate your protein. These include:

Centrifugation (filtration) using concentrators
Dialysis
Lyophilization
Precipitation

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Concentrador de proteínas de PES Pierce™, MWCO de 30 K, de 5 a 20 ml FAQs

Citations & References (4)

Citations & References
Abstract
Glucocorticoid stress hormones stimulate vesicle-free Tau secretion and spreading in the brain.
Authors:Yu Q,Du F,Belli I,Gomes PA,Sotiropoulos I,Waites CL
Journal:bioRxiv : the preprint server for biology
PubMed ID:37333306
Chronic stress and elevated levels of glucocorticoids (GCs), the main stress hormones, accelerate Alzheimer's disease (AD) onset and progression. A major driver of AD progression is the spreading of pathogenic Tau protein between brain regions, precipitated by neuronal Tau secretion. While stress and high GC levels are known to induce ... More
DNA sequence-induced solid phase transition as a solution to the genome folding paradox.
Authors:Pulupa JM,McArthur NG,Stathi O,Wang M,Zazhytska M,Pirozzolo ID,Nayar A,Shapiro L,Lomvardas S
Journal:Research square
PubMed ID:39678350
Ultra long-range genomic contacts, which emerge as prominent components of genome architecture, constitute a biochemical paradox. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located megabases away, instead of interacting with proximal sequences occupied by the same exact transcription factors (TF). This is exemplified ... More
Noncompetitive immunoassay optimized for pharmacokinetic assessments of biologically active efruxifermin.
Authors:Kinne AS,Tillman EJ,Abdeen SJ,Johnson DE,Parmer ES,Hurst JP,de Temple B,Rinker S,Rolph TP,Bowsher RR
Journal:Journal of pharmaceutical and biomedical analysis
PubMed ID:37141854
Efruxifermin (EFX) is a homodimeric human IgG(1) Fc-FGF21 fusion protein undergoing investigation for treatment of liver fibrosis due to nonalcoholic steatohepatitis (NASH), a prevalent and serious metabolic disease for which there is no approved treatment. Biological activity of FGF21 requires its intact C-terminus, which enables binding to its obligate co-receptor ... More
Antigenic drift expands influenza viral escape pathways from recalled humoral immunity
Authors:Maurer DP, Vu M, Schmidt AG.
Journal:Immunity
PubMed ID:40023162
Initial exposure to a rapidly evolving virus establishes B cell memory that biases later responses to antigenically drifted strains. This "immune imprinting" implies that subsequent exposure to a drifted strain can induce affinity maturation of memory B cells toward cross-reactivity with the drifted strain and hence toward greater overall breadth. ... More