Medios BG-11 optimizados para cianobacterias
Medios BG-11 optimizados para cianobacterias
Gibco™

Medios BG-11 optimizados para cianobacterias

Los medios Gibco™ BG-11 están optimizados para el crecimiento y mantenimiento de cianobacterias seleccionadas, lo que incluye Synechococcus elongatus. LaMás información
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Número de catálogoCantidad
A13799011 L
A13799026 L
Número de catálogo A1379901
Precio (EUR)
69,75
Each
Añadir al carro de la compra
Cantidad:
1 L
Precio (EUR)
69,75
Each
Añadir al carro de la compra
Los medios Gibco™ BG-11 están optimizados para el crecimiento y mantenimiento de cianobacterias seleccionadas, lo que incluye Synechococcus elongatus. La formulación está lista para su uso en 1X para evitar laboriosos pasos de preparación de medios.

Cada medio Gibco™ BG-11 se presenta en el premiado frasco Gibco™ diseñado para un uso más fácil en la cabina de bioseguridad, lo que minimiza el riesgo de contaminación y le ayuda a realizar el cultivo celular de forma más uniforme. En conjunto, el embalaje y la calidad superiores, la mayor fiabilidad y la uniformidad mejorada en el cultivo de cianobacterias dan como resultado una mayor eficacia global y datos más sólidos (fig. 1, 2).

Para uso exclusivo en investigación. No diseñado para uso diagnóstico o terapéutico en humanos ni en animales.
Especificaciones
Tipo de mediosMedios de algas
Método de preparaciónNinguno (listo para usar)
Cantidad1 L
Condiciones de envíoTemperatura ambiente
FormularioLíquido
Tipo de productoMedia Broth
Clase de organismo dianaCianobacterias
Unit SizeEach
Contenido y almacenamiento
Los medios de crecimiento Gibco™ BG-11 contienen:

•  1 l de medios Gibco™ BG-11; almacenar a 4 °C

Preguntas frecuentes

I'm getting low basal expression of my gene of interest when using the pSyn_1 vector. What do you suggest I try?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pSyn_1 and pSyn_6?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the shelf-life/competency of Synechococcus?

After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the mechanism of integration when using the Synechococcus expression kits?

A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the strain of Synechococcus used in your kits?

We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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