Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium (A1517001), vitronectin (VTN-N) has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. In addition, vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Guokai Chen et al. (1) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8™ Medium.
Vitronectin (VTN-N) is:
• Optimized—designed in the laboratory of James Thomson for use with Essential 8™ Medium • Defined—for reduced variability in your PSC cultures • Cost effective—for economical and scalable PSC culture
Optimized and Defined Surface Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) was designed in the laboratory of James Thomson for use with Essential 8™ Medium and has been shown to support human pluripotent stem cell attachment and survival better than wild type vitronectin. Vitronectin (VTN-N) provides an optimal environment for feeder-free pluripotent stem cell culture.
Reduce Variability Vitronectin (VTN-N) is a defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs).
Cost Effective When used with Essential 8™ Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder free culture of human pluripotent stem cells (PSCs).
Commercialized in Partnership with Cellular Dynamics International.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Concentración
500 μg⁄ml
Para utilizar con (aplicación)
Stem Cell Culture
Método de purificación
HPLC
Cantidad
1 mL
Especie
Human
Recombinante
Recombinant
Clasificación
AOF
Nivel de endotoxinas
Very Low
Condiciones de envío
Dry ice
Volumen (métrico)
1 mL
Forma de proteína
Recombinant
Formulario
Liquid
Subtipo de proteína
Vitronectin
Categoría de investigación
Stem Cell Research
Grado de pureza o calidad
95 %
Contenido y almacenamiento
Store at -80°C and protect from light.
Figuras
Documentos y descargas
Certificados
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